The Jackson Laboratory, Bar Harbor, ME, USA.
Department of Molecular Haematology, Norwich Medical School, University of East Anglia, Norwich, UK.
Methods Mol Biol. 2023;2675:43-49. doi: 10.1007/978-1-0716-3247-5_4.
In vivo imaging enables the detection and visualization of many different processes occurring within the body. Fatty acid uptake is a fundamental cellular process which is essential for the use of free fatty acids (FFAs) as a fuel source for metabolism. Detection and visualization of in vivo FFA uptake in the bone marrow has been relatively unknown. Here, we describe the process of non-invasive bioluminescent imaging of in vivo FFA uptake within the bone marrow.
体内成像是一种可以检测和可视化体内许多不同过程的方法。脂肪酸摄取是一种基本的细胞过程，对于将游离脂肪酸（FFAs）用作代谢燃料来源至关重要。在骨髓中检测和可视化体内 FFA 摄取的方法一直鲜为人知。在这里，我们描述了在骨髓中进行非侵入性生物发光成像以检测和可视化体内 FFA 摄取的过程。
