The Department of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qing Chun Road, Hangzhou, 310006, China.
Virol J. 2023 May 31;20(1):107. doi: 10.1186/s12985-023-02078-z.
Patients infected with HIV are at high risk of developing Epstein-Barr Virus (EBV)-related diseases. The genotype and viral biological behavior of EBV infection in patients with human immunodeficiency virus-1 (HIV) in China remain unclear. This study analyzed the characteristics of EBV in patients infected with HIV in southeastern China.
A total of 162 HIV-infected patients and 52 patients without HIV were enrolled in this study. EBV viral load in blood was determined by fluorescence quantitative PCR. EBV typing was performed using saliva according to polymorphisms in the EBNA3C region. EBV LMP-1 carboxy terminus (C-ter) was sequenced, and compared with the epidemic strains in the world.
Among HIV infected patients, the EBV strain variant was mainly EBV-1, while EBV-2 had a higher viral load than EBV-1 (P = 0.001) and EBV-1/2 (P = 0.002). HIV infected patients had higher active virus replication. The EBV LMP-1 variants were mainly the China1 variant. HIV-infected patients had different nucleic acid positions of 30-bp deletion (del30) and had a higher incidence of high 33-bp tandem repeats (rep33) copies than non-HIV-infected patients. There was a difference in the mutations of EBV LMP-1 C-ter del30 and ins15 between HIV infected patients and the control group (P < 0.001).
In southeastern China, EBV in HIV-infected patients had higher active virus replication; EBV infection was mainly EBV-1, and EBV-2 infection has higher EBV virus load; hotspot mutations of LMP-1 C-ter were different between HIV-infected patients and non-HIV-infected patients.
This study was approved by the ethics committee of the First Affiliated Hospital of Zhejiang University School of Medicine (Approval No. 2018764), and registered in Chinese Clinical Trial Registry on 3 June 2019 (ChiCTR, ChiCTR1900023600, http://www.chictr.org.cn/usercenter.aspx ).
感染 HIV 的患者发生 EBV 相关疾病的风险较高。中国 HIV-1 感染者 EBV 感染的基因型和病毒生物学行为尚不清楚。本研究分析了中国东南部 HIV 感染者 EBV 的特征。
本研究共纳入 162 例 HIV 感染者和 52 例非 HIV 感染者。采用荧光定量 PCR 检测血液 EBV 病毒载量，根据 EBNA3C 区多态性进行 EBV 定型，对 EBV LMP-1 羧基末端（C 端）进行测序，并与世界流行株进行比较。
在 HIV 感染者中，EBV 株变异主要为 EBV-1，而 EBV-2 的病毒载量高于 EBV-1（P=0.001）和 EBV-1/2（P=0.002）。HIV 感染者存在更高的病毒复制活性。EBV LMP-1 变异主要为 China1 变异株，HIV 感染者 EBV LMP-1 C 端 30-bp 缺失（del30）核酸位置不同，高 33-bp 串联重复（rep33）拷贝数的发生率高于非 HIV 感染者。HIV 感染者 EBV LMP-1 C 端 del30 和 ins15 的突变与对照组存在差异（P<0.001）。
在中国东南部，HIV 感染者 EBV 具有更高的病毒复制活性；EBV 感染以 EBV-1 为主，而 EBV-2 感染具有更高的 EBV 病毒载量；LMP-1 C 端热点突变在 HIV 感染者和非 HIV 感染者之间存在差异。
本研究经浙江大学医学院附属第一医院伦理委员会批准（批准号：2018764），并于 2019 年 6 月 3 日在中国临床试验注册中心注册（ChiCTR，ChiCTR1900023600，http://www.chictr.org.cn/usercenter.aspx）。
