分析中国儿童 EB 病毒感染相关疾病中 EBNA-1 和 LMP-1 变异体。
Analysis of EBNA-1 and LMP-1 variants in diseases associated with EBV infection in Chinese children.
机构信息
Key Laboratory of Major Diseases in Children, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing 100045, China.
出版信息
Virol J. 2012 Jan 11;9:13. doi: 10.1186/1743-422X-9-13.
BACKGROUND
In China, primary EBV infection occurs during childhood with seroprevalence reaching about 100% by 10 years of age. There are few studies on EBV variants in diseases associated with EBV infection in Chinese children. In this study, we investigated the diversity of the EBV genes (EBNA-1 and LMP-1) and the relationship between EBV variants and the clinical phenotypes in diseases associated with EBV infections in Chinese pediatric cases.
RESULTS
The frequencies of EBV type I in the IM, HLH and HL samples were 98.4%, 100% and 95.8%, respectively. Three known EBNA-1 variants were identified, including V-val (all were V-val-v1 sub-variant), P-thr' and V-Leu (MT). The frequency of V-val-v1 was 98.6% in the IM samples, 100% in the HLH samples and 97.1% in the HL samples. There were no significant differences of the distribution of EBNA-1 variants between IM, HLH and HL samples (P > 0.05). Three known LMP-1 variants, including China 1, China 2 and Med, were identified and China 1 was predominant in all groups (IM 88.6%, HLH 100% and HL 100%). The frequency of del-LMP-1 was 88.6% in the IM samples, 100% in the HLH samples and 96.0% in the HL samples. There were no significant differences in the frequency of del-LMP-1 between the IM, HLH and HL samples (P > 0.05). The frequency of XhoI loss was 90.6% in the IM samples, 100% in the HLH samples and 100% in the HL samples, with no significant difference in frequency (P > 0.05). In the EBV type I strain, V-val-v1 variant (EBNA-1) was linked with China1 variant (LMP-1) in 88.9% of the IM samples, 100% of the HLH samples and 80.0% of the HL samples in this study.
CONCLUSIONS
Type I EBV was the most prevalent subtype EBV in Chinese pediatric cases and V-val-v1 (EBNA-1) and China1 (LMP-1) variants were the most dominant variants. There was a strong linkage between V-val-v1 (EBNA-1) variant and China1 (LMP-1) variant in type I EBV. The sequence variation in EBV genes may represent a geographic polymorphism since no preferential associations were found between specific EBV variants and specific diseases in this study.
背景
在中国，原发性 EBV 感染发生在儿童期，到 10 岁时血清阳性率达到约 100%。关于与 EBV 感染相关疾病中 EBV 变异株的研究较少。本研究旨在调查 EBNA-1 和 LMP-1 基因的 EBV 变异株多样性以及与中国儿科病例中与 EBV 感染相关疾病的临床表型之间的关系。
结果
在 IM、HLH 和 HL 样本中，EBV Ⅰ型的频率分别为 98.4%、100%和 95.8%。鉴定出三种已知的 EBNA-1 变异株，包括 V-val（均为 V-val-v1 亚变异株）、P-thr'和 V-Leu（MT）。在 IM 样本中，V-val-v1 的频率为 98.6%，在 HLH 样本中为 100%，在 HL 样本中为 97.1%。IM、HLH 和 HL 样本中 EBNA-1 变异株的分布无显著性差异（P>0.05）。鉴定出三种已知的 LMP-1 变异株，包括 China1、China2 和 Med，所有组均以 China1 为主（IM 88.6%、HLH 100%和 HL 100%）。在 IM 样本中，del-LMP-1 的频率为 88.6%，在 HLH 样本中为 100%，在 HL 样本中为 96.0%。IM、HLH 和 HL 样本中 del-LMP-1 的频率无显著性差异（P>0.05）。XhoI 缺失的频率在 IM 样本中为 90.6%，在 HLH 样本中为 100%，在 HL 样本中为 100%，频率无显著性差异（P>0.05）。在 EBV Ⅰ型株中，V-val-v1 变异株（EBNA-1）与 China1 变异株（LMP-1）在本研究的 88.9%的 IM 样本、100%的 HLH 样本和 80.0%的 HL 样本中存在关联。
结论
在中国儿科病例中，Ⅰ型 EBV 是最常见的 EBV 亚型，V-val-v1（EBNA-1）和 China1（LMP-1）变异株是最主要的变异株。Ⅰ型 EBV 中 V-val-v1（EBNA-1）变异株与 China1（LMP-1）变异株之间存在较强的连锁关系。EBV 基因的序列变异可能代表一种地理多态性，因为在本研究中，没有发现特定 EBV 变异株与特定疾病之间存在优先关联。
Zotero 插件