Gantuz Magdalena, Lorenzetti Mario Alejandro, Chabay Paola Andrea, Preciado María Victoria
Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas (IMIPP), CONICET-GCBA, Laboratorio de Biología Molecular, División Patología, Hospital de Niños Ricardo Gutiérrez, Buenos Aires, Argentina.
PLoS One. 2017 Mar 22;12(3):e0174221. doi: 10.1371/journal.pone.0174221. eCollection 2017.
To study LMP1 variants distribution among children with EBV+ malignant and benign conditions as well as in healthy carriers.
Oral secretions and blood cells from 31 children with IM, and biopsies from 14 EBV+ reactive lymphoid hyperplasia and 33 EBV+ lymphomas were included. LMP1 was amplified by nested PCR and sequenced. Phylogenetic reconstructions were made under Maximun Likelihood, Bayesian and coalescent algorithms.
Six clades were defined (China1, China2, Med-, Alaskan, B95.8 and Argentine). Argentine variants, the most prevalent (46%), harbored 3 distinctive mutations and were a recombination between Raji and China1. Despite no pathology or compartment associations were observed for LMP1, the Argentine clade showed a phylogeographic association with our region. LMP1 estimated evolution rate was 8.591x10-5s/s/y and the estimated tMRCA for Raji and Argentine was 136ybp.
An LMP1 Argentine clade was defined. LMP1 evolutionary rate was higher than expected for herpesviruses. The tMRCA for Raji and the Argentine agrees with African immigration and could explain the recombinant nature of the Argentine variant.
研究LMP1变体在EBV阳性的恶性和良性疾病儿童以及健康携带者中的分布情况。
纳入31例传染性单核细胞增多症儿童的口腔分泌物和血细胞，以及14例EBV阳性反应性淋巴组织增生和33例EBV阳性淋巴瘤的活检组织。通过巢式PCR扩增LMP1并进行测序。在最大似然法、贝叶斯法和溯祖算法下进行系统发育重建。
定义了六个进化枝（中国1、中国2、地中海型、阿拉斯加型、B95.8型和阿根廷型）。阿根廷型变体最为常见（46%），有3个独特的突变，是Raji型和中国1型之间的重组体。尽管未观察到LMP1与病理或组织区室的关联，但阿根廷进化枝与我们所在地区存在系统地理学关联。LMP1估计进化速率为8.591×10-5秒/位点/年，Raji型和阿根廷型的估计最近共同祖先时间为136年前。
定义了LMP1阿根廷进化枝。LMP1的进化速率高于疱疹病毒的预期。Raji型和阿根廷型的最近共同祖先时间与非洲移民情况相符，这可以解释阿根廷型变体的重组性质。
