Taşkan Tuba, Turan Taylan, İltemir Duvan Zehra Candan, Gönenç Aymelek
Afyonkarahisar Health Sciences University Faculty of Pharmacy, Department of Biochemistry, Afyonkarahisar, Turkey.
Gazi University Faculty of Pharmacy, Department of Biochemistry, Ankara, Turkey.
Turk J Obstet Gynecol. 2023 Jun 1;20(2):113-119. doi: 10.4274/tjod.galenos.2023.87936.
Unexplained infertility refers to a diagnosis in which all standard examinations are usually normal and is statistically seen in approximately 30-40% of infertile couples and endometriosis encountered in 25-50% of patients with unexplained infertility. Unexplained infertility is thought to be closely associated with endometriosis and serum and peritoneal fluid levels of Fetuin-A is increased in patients with endometriosis. Fetuin-A is proposed as a diagnostic marker for endometriosis and has anti-inflammatory effects on several diseases. Oxidative stress also is central to the etiopathogenesis of infertility in women. The aim of this study was to evaluate serum Fetuin-A and oxidative stress parameter concentrations impact on unexplained infertility.
In the study, serum Fetuin-A, IL-1β, CA I, TAS, TOS levels, and PON and ARE enzyme activities were measured using the Enzyme-Linked ImmunoSorbent Assay in the sera of diagnosed unexplained infertility females (n=44) and controls (n=41).
There was no statistically significant difference between unexplained infertile patients and control groups in terms of serum IL-1β, CA I, OSI, and PON levels (p>0.05). Serum Fetuin-A and ARE levels were significantly higher in unexplained infertility compared with the control, whereas serum TAS and TOS levels were lower (p<0.05, p<0.01, p<0.05, p<0.05, respectively).
It is thought that increased Fetuin-A levels may be a response to the inflammatory process and increased ARE activity may be a sign of the impaired oxidant-antioxidant balance in unexplained infertility. This may contribute to the pathogenesis of infertility, and the data obtained will make a significant contribution to new works to be done in this sense.
不明原因不孕症是指所有标准检查通常均正常的一种诊断，据统计约30%-40%的不孕夫妇患有此病，而在25%-50%的不明原因不孕症患者中会出现子宫内膜异位症。不明原因不孕症被认为与子宫内膜异位症密切相关，子宫内膜异位症患者血清和腹水中胎球蛋白-A水平升高。胎球蛋白-A被提议作为子宫内膜异位症的诊断标志物，并且对多种疾病具有抗炎作用。氧化应激在女性不孕症的发病机制中也起着核心作用。本研究的目的是评估血清胎球蛋白-A和氧化应激参数浓度对不明原因不孕症的影响。
在本研究中，采用酶联免疫吸附测定法测量了确诊为不明原因不孕症的女性（n=44）和对照组（n=41）血清中的胎球蛋白-A、白细胞介素-1β、碳酸酐酶I、总抗氧化状态、总氧化状态水平，以及对氧磷酶和芳烃羟化酶的活性。
不明原因不孕患者与对照组在血清白细胞介素-1β、碳酸酐酶I、氧化应激指数和对氧磷酶水平方面无统计学显著差异（p>0.05）。与对照组相比，不明原因不孕症患者血清胎球蛋白-A和芳烃羟化酶水平显著更高，而血清总抗氧化状态和总氧化状态水平更低（分别为p<0.05、p<0.01、p<0.05、p<0.05）。
认为胎球蛋白-A水平升高可能是对炎症过程的一种反应，而芳烃羟化酶活性增加可能是不明原因不孕症中氧化-抗氧化平衡受损的一个迹象。这可能有助于不孕症的发病机制，并且所获得的数据将对在这方面开展的新研究做出重大贡献。
