Key Laboratory of Crop Epigenetic Regulation and Development in Hunan Province, Hunan Agricultural University, Changsha, Hunan, China.
College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan, China.
Physiol Plant. 2023 May-Jun;175(3):e13944. doi: 10.1111/ppl.13944.
Chlorogenic acid is a key chemical in antioxidation and antisepsis. Sambucus chinensis L. is an herbaceous plant rich in chlorogenic acid and a potential genetic resource for breeding high-chlorogenic acid plants. However, there are few studies on the synthesis pathway of chlorogenic acid in S. chinensis. Our study found chlorogenic acid accumulation in S. chinensis to be organ-specific, higher in leaves and buds but lower in roots, stems and fruits. A total number of 546,844 CCS (circular consensus sequence), including 402,767 full-length nonchimeric (FLNC) and 39 annotated sequences related to the synthesis of chlorogenic acid, was obtained by single-molecule real-time sequencing technology (SMRT). qRT-PCR showed that a number of key genes involved in chlorogenic acid synthesis were differentially expressed in various tissues of S. chinensis. Transgenic tobacco revealed that ectopic expression of the HCT homologous gene HCT-45178 increased the content of chlorogenic acid. Our results should be the first report of full-length transcriptome data of S. chinensis, which help to understand the basis of chlorogenic acid synthesis and provide a novel strategy for breeding tobacco cultivars with higher levels of chlorogenic acid.
绿原酸是抗氧化和防腐的关键化学物质。忍冬是一种富含绿原酸的草本植物，是培育高绿原酸植物的潜在遗传资源。然而，关于忍冬中绿原酸的合成途径的研究较少。我们的研究发现，绿原酸在忍冬中的积累具有器官特异性，在叶和芽中较高，但在根、茎和果实中较低。通过单分子实时测序技术（SMRT）获得了 546844 个 CCS（圆形一致序列），包括 402767 个全长非嵌合（FLNC）和 39 个与绿原酸合成相关的注释序列。qRT-PCR 显示，在忍冬的不同组织中，一些参与绿原酸合成的关键基因表达水平存在差异。转基因烟草显示，异位表达 HCT 同源基因 HCT-45178 增加了绿原酸的含量。我们的研究结果应该是忍冬全长转录组数据的首次报道，有助于理解绿原酸合成的基础，并为培育绿原酸含量更高的烟草品种提供了新的策略。
