Sheraz Ahmad, Zhu Haowen, Dong Qiaoqiao, Wang Tingting, Zong Suman, Wang Huaiqi, Ge Linquan, Wu Tao
College of Plant Protection, Yangzhou University, Yangzhou, China.
College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou, China.
Front Physiol. 2023 May 10;14:1197395. doi: 10.3389/fphys.2023.1197395. eCollection 2023.
Brown planthopper (BPH), Stål (Hemiptera: Delphacidae), is a major rice pest causing significant damage to rice throughout the world. Intensive pesticide usage often causes resistance in these seasonal pests, mainly through the modulation of antioxidant machinery. The superoxide dismutase (SOD) gene family is known for regulating BPH response to pesticides. In the present study, we identified eight genes from the NCBI using the BLASTP program. The bioinformatics analysis includes a phylogenetic tree, conserved domain, motifs, gene ontology (GO) analysis, Kyoto encyclopedia of genes and genomes (KEGG) pathways, and protein-protein interaction, highlighting the distinctive functional elements of genes. Additionally, the genes showed expression in all developmental stages of BPH. Under three sugars (glucose, sucrose, and trehalose) treatment, the respective upregulation of and was noted. The induced significantly under jinggamycin (JGM) deduced its potential as a key regulator of BPH response to the pesticide. Our study has provided detailed knowledge of the gene family in-silico analysis and the defensive response to insecticide and high sugar of BPH. We hope the results of this research will help to shed light on the resistance of BPH towards insecticide toxicity and high sugar and help to control it more efficiently.
褐飞虱（BPH），斯塔尔（半翅目：飞虱科），是一种主要的水稻害虫，在全球范围内对水稻造成重大损害。大量使用农药往往会导致这些季节性害虫产生抗性，主要是通过调节抗氧化机制。超氧化物歧化酶（SOD）基因家族以调节褐飞虱对农药的反应而闻名。在本研究中，我们使用BLASTP程序从NCBI中鉴定出八个基因。生物信息学分析包括系统发育树、保守结构域、基序、基因本体（GO）分析、京都基因与基因组百科全书（KEGG）途径以及蛋白质-蛋白质相互作用，突出了基因独特的功能元件。此外，这些基因在褐飞虱的所有发育阶段均有表达。在三种糖类（葡萄糖、蔗糖和海藻糖）处理下，分别观察到了和的上调。井冈霉素（JGM）处理下显著诱导，推断其作为褐飞虱对该农药反应的关键调节因子的潜力。我们的研究提供了关于褐飞虱基因家族的详细的计算机分析知识以及对杀虫剂和高糖的防御反应。我们希望这项研究的结果将有助于阐明褐飞虱对杀虫剂毒性和高糖的抗性，并有助于更有效地控制它。
