Yamamoto Kohji, Yamaguchi Misuzu
Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University Graduate School, Fukuoka, Japan.
Arch Insect Biochem Physiol. 2022 Feb;109(2):e21862. doi: 10.1002/arch.21862. Epub 2021 Dec 12.
The brown planthopper (Nilaparvata lugens) is a major agricultural pest of rice crops. Analysis of the enzymes produced by N. lugens is important to develop pest-control methods. Superoxide dismutase (SOD) is a detoxification enzyme that catalyzes the conversion of superoxide anions (reactive oxygen species) into oxygen and hydrogen peroxide. As there have been no reports on SOD in N. lugens, in this study, we characterized a new SOD in the brown planthopper, nlSOD1. Amino acid sequence and phylogenetic analyses revealed that nlSOD1 is a member of the Cu/Zn-SOD family. Recombinant nlSOD1, when overexpressed in Escherichia coli, catalyzes the dismutation of superoxide radicals into molecular O and H O . Exposure to various insecticides induced nlSOD1 messenger RNA expression. These results indicate that nlSOD1 may contribute to the insecticide resistance of N. lugens. The findings of this study may assist in the development of novel methods to control the population of N. lugens.
褐飞虱（Nilaparvata lugens）是水稻作物的主要农业害虫。分析褐飞虱产生的酶对于开发害虫防治方法很重要。超氧化物歧化酶（SOD）是一种解毒酶，可催化超氧阴离子（活性氧）转化为氧气和过氧化氢。由于尚未有关于褐飞虱中SOD的报道，在本研究中，我们鉴定了褐飞虱中的一种新SOD，即nlSOD1。氨基酸序列和系统发育分析表明，nlSOD1是铜/锌-SOD家族的成员。重组nlSOD1在大肠杆菌中过表达时，可催化超氧自由基歧化为分子氧和过氧化氢。暴露于各种杀虫剂会诱导nlSOD1信使RNA表达。这些结果表明，nlSOD1可能有助于褐飞虱对杀虫剂的抗性。本研究结果可能有助于开发控制褐飞虱种群的新方法。
