Key Laboratory of Green Chemistry and Technology of Ministry of Education, College of Chemistry, Sichuan University, 29, Wangjiang Road, Chengdu 610064, P. R. China.
ACS Sens. 2023 Jun 23;8(6):2359-2367. doi: 10.1021/acssensors.3c00470. Epub 2023 Jun 2.
Accurate detection of target analytes and generation of high-fidelity fluorescence signals are particularly critical in life sciences and clinical diagnostics. However, the majority of current NIR-I fluorescent probes are vulnerable to pH effects resulting in signal distortion. In this work, a series of fluorescence-tunable and pH-independent probes are reported by combining optically tunable groups of unsymmetric Si-rhodamines and introducing the methoxy instead of the spiro ring on the benzene ring at position 9. To validate the concept, the leucine aminopeptidase response site was introduced into with the best optical properties to synthesize for monitoring the intrahepatic LAP in vivo. Therefore, the design approach may provide a new and practical strategy for designing innovative functional fluorescent probes and generating high-stability and high-fidelity fluorescent signals.
准确检测目标分析物并产生高保真荧光信号在生命科学和临床诊断中尤为关键。然而，目前大多数近红外 I 区荧光探针易受 pH 影响，导致信号失真。在这项工作中，通过将不对称硅罗丹明的光可调基团组合，并在苯环 9 位引入甲氧基而不是螺环，报道了一系列荧光可调且与 pH 无关的探针。为了验证这一概念，将亮氨酸氨肽酶反应位点引入具有最佳光学性能的 中，以合成 用于监测体内肝内 LAP。因此，该设计方法可能为设计创新的功能荧光探针和产生高稳定性和高保真荧光信号提供一种新的实用策略。
