Wei Yicong, Zhang Jiayuan, Qi Keming, Li Ye, Chen Ying
College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China.
College of Landscape and Architecture, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Chin Herb Med. 2022 Nov 21;15(2):298-309. doi: 10.1016/j.chmed.2022.11.002. eCollection 2023 Apr.
Diterpenoids with a wide variety of biological activities from , a precious medicinal plant, are important active components However, due to the lack of genetic information on the metabolic process of diterpenoids in , the genes involved in the molecular regulation mechanism of diterpenoid metabolism are still unclear. This study revealed the complex metabolic genes for diterpenoids biosynthesis in different organs of by combining analysis of transcriptomics and metabolomics.
The differences in diterpenoid accumulation in roots, stems and leaves of were analyzed by metabonomic analysis, and its metabolic gene information was obtained by transcriptome sequencing. Then, the molecular mechanism of differential diterpenoid accumulation in different organs of was analyzed from the perspective of gene expression patterns.
A total of 296 terpenoid metabolites were identified in the five terpenoid metabolic pathways in . There were 38, 34, and 18 diterpenoids with different contents between roots and leaves, between leaves and stems, and between roots and stems, respectively. Twenty-nine metabolic enzyme genes with 883 unigenes in the diterpenoid synthesis process were identified, and the DXS and FDPS in the terpenoid backbone biosynthesis stage and CPA, GA20ox, GA3ox, GA2ox, and MAS in the diterpenoid biosynthesis stage were predicted to be the key metabolic enzymes for the accumulation of diterpenoids. In addition, 14 key transcription factor coding genes were predicted to be involved in the regulation of the diterpenoid biosynthesis. The expression of genes such as , , , and might be activated by some of the 14 transcription factors. The transcription factor NTF-Y and PRE6 were predicted to be the most important transcription factors.
This study determined 29 metabolic enzyme genes and predicted 14 transcription factors involved in the molecular regulation mechanism of diterpenoid metabolism in , which provided a reference for the further study of the molecular regulation mechanism of the accumulation of diterpenoids in different organs of .
作为一种珍贵的药用植物，[植物名称]含有具有多种生物活性的二萜类化合物，它们是重要的活性成分。然而，由于缺乏[植物名称]中二萜类化合物代谢过程的遗传信息，参与二萜类化合物代谢分子调控机制的基因仍不清楚。本研究通过转录组学和代谢组学分析相结合，揭示了[植物名称]不同器官中二萜类化合物生物合成的复杂代谢基因。
通过代谢组学分析[植物名称]根、茎、叶中二萜类化合物积累的差异，并通过转录组测序获得其代谢基因信息。然后，从基因表达模式的角度分析[植物名称]不同器官中二萜类化合物差异积累的分子机制。
在[植物名称]的五条萜类代谢途径中共鉴定出296种萜类代谢物。根与叶、叶与茎、根与茎之间分别有38种、34种和18种含量不同的二萜类化合物。在二萜类化合物合成过程中鉴定出29个代谢酶基因，包含883个单基因，并且预测萜类骨架生物合成阶段的DXS和FDPS以及二萜类化合物生物合成阶段的CPA、GA20ox、GA3ox、GA2ox和MAS是二萜类化合物积累的关键代谢酶。此外，预测有14个关键转录因子编码基因参与二萜类化合物生物合成的调控。[基因名称1]、[基因名称2]、[基因名称3]、[基因名称4]和[基因名称5]等基因的表达可能被这14个转录因子中的一些激活。转录因子NTF-Y和PRE6被预测为最重要的转录因子。
本研究确定了29个参与[植物名称]二萜类化合物代谢分子调控机制的代谢酶基因，并预测了14个转录因子，为进一步研究[植物名称]不同器官中二萜类化合物积累的分子调控机制提供了参考。
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