Sun Meng, Ma Qian, Wang Xueqi, Guo Jialiang, Wang Jiaxuan, Zhang Dongrui, Tkachenko Kirill, Wang Wenzhong, Chang Ying
College of Life Sciences, Northeast Agricultural University, Harbin 150030, China.
Peter the Great Botanical Garden, V.L. Komarov Botanical Institute of the Russian Academy of Sciences, Saint-Petersburg 197376, Russia.
Plants (Basel). 2025 Jul 15;14(14):2190. doi: 10.3390/plants14142190.
(L.) Schott synthesizes volatile sesquiterpenes through the mevalonate pathway (MVA), in which 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) serves as the key rate-limiting enzyme. Although HMGR plays a crucial role in terpenoid biosynthesis, its functional characteristics in and its involvement in stress responses remain unclear. This study identified three genes () from the transcriptome data of . Bioinformatics analysis revealed that the encoded proteins are localized to the endoplasmic reticulum and share high sequence similarity with fern homologs. Under abiotic stress conditions, s exhibited differential expression patterns, with marked upregulation under salt and drought stress. To validate the functions of these genes, we generated transgenic L. plants overexpressing s. Compared with wild-type controls, the transgenic lines showed enhanced tolerance to drought and heat stress. Promoter analysis identified functional regulatory regions controlling expression, and co-expression network analysis predicted 21 potential transcriptional regulators. This study validates the function of in a heterologous system and provides candidate genes for improving stress resistance in plants.
（L.）肖特通过甲羟戊酸途径（MVA）合成挥发性倍半萜，其中3-羟基-3-甲基戊二酰辅酶A还原酶（HMGR）是关键的限速酶。尽管HMGR在萜类生物合成中起关键作用，但其在[植物名称未给出]中的功能特性及其参与应激反应的情况仍不清楚。本研究从[植物名称未给出]的转录组数据中鉴定出三个[基因名称未给出]基因。生物信息学分析表明，编码的蛋白质定位于内质网，与蕨类植物同源物具有高度序列相似性。在非生物胁迫条件下，[基因名称未给出]表现出差异表达模式，在盐胁迫和干旱胁迫下显著上调。为了验证这些基因的功能，我们构建了过表达[基因名称未给出]的转基因[植物名称未给出]植株。与野生型对照相比，转基因株系对干旱和热胁迫的耐受性增强。启动子分析确定了控制[基因名称未给出]表达的功能调控区域，共表达网络分析预测了21个潜在的转录调节因子。本研究在异源系统中验证了[基因名称未给出]的功能，并为提高植物抗逆性提供了候选基因。
