Gao Hongyang, Zhang Huiju, Hu Yuqing, Xu Danyun, Zheng Sikai, Su Shuting, Yang Quan
School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Key Laboratory of Production & Development of Cantonese Medicinal Materials, State Administration of Traditional Chinese Medicine, Guangzhou 510006, China.
Chin Herb Med. 2023 Feb 16;15(2):310-316. doi: 10.1016/j.chmed.2022.06.013. eCollection 2023 Apr.
The barks, leaves, and branches of have been historically used as a traditional Chinese medicine, spice, and food preservative, in which phenylpropanoids are responsible compounds. However phenylpropanoid biosynthesis pathways are not clear in . We elucidated the pathways by descriptive analyses of differentially expressed genes related to phenylpropanoid biosynthesis as well as to identify various phenylpropanoid metabolites.
Chemical analysis, metabolome sequencing, and transcriptome sequencing were performed to investigate the molecular mechanisms underlying the difference of active components content in the barks, branches and leaves of .
Metabolomic analysis revealed that small amounts of flavonoids, coumarine, and cinnamaldehyde accumulated in both leaves and branches. Transcriptome analysis showed that genes associated with phenylpropanoid and flavonoid biosynthesis were downregulated in the leaves and branches relative to the barks. The observed differences in essential oil content among the three tissues may be attributable to the differential expression of genes involved in the phenylpropanoid and flavonoid metabolic pathways.
This study identified the key genes in the phenylpropanoid pathway controling the flavonoid, coumarine, and cinnamaldehyde contents in the barks, branches and leaves by comparing the transcriptome and metabolome These findings may be valuable in assessing phenylpropanoid and flavonoid metabolites and identifying specific candidate genes that are related to the synthesis of phenylpropanoids and flavonoids in .
[植物名称]的树皮、树叶和树枝在历史上一直被用作传统中药、香料和食品防腐剂，其中苯丙素类化合物是起作用的成分。然而，[植物名称]中苯丙素生物合成途径尚不清楚。我们通过对与苯丙素生物合成相关的差异表达基因进行描述性分析，阐明了这些途径，并鉴定了各种苯丙素代谢产物。
进行化学分析、代谢组测序和转录组测序，以研究[植物名称]树皮、树枝和树叶中活性成分含量差异背后的分子机制。
代谢组分析表明，树叶和树枝中积累了少量黄酮类化合物、香豆素和肉桂醛。转录组分析表明，相对于树皮，参与苯丙素和黄酮生物合成的基因在树叶和树枝中表达下调。在这三个组织中观察到的精油含量差异可能归因于参与苯丙素和黄酮代谢途径的基因的差异表达。
本研究通过比较转录组和代谢组，确定了苯丙素途径中控制树皮、树枝和树叶中黄酮类化合物、香豆素和肉桂醛含量的关键基因。这些发现可能有助于评估苯丙素和黄酮类代谢产物，并鉴定与[植物名称]中苯丙素和黄酮类化合物合成相关的特定候选基因。
