Teng Shuai, Zhu Zhaowei, Li Yang, Hu Xinqun, Fang Zhenfei, Liu Zhenjiang, Zhou Shenghua
Department of Cardiovascular Medicine, the Second Xiangya Hospital, Central South University, Changsha, Hunan, China.
Department of Vascular Surgery, Xiamen Cardiovascular Hospital, Xiamen University, Xiamen, Fujian, China.
Front Pharmacol. 2023 May 17;14:1159779. doi: 10.3389/fphar.2023.1159779. eCollection 2023.
Most drug-eluting stents (DESs) inhibit intimal hyperplasia but impair re-endothelialization. This study aimed to evaluate in vivo strut coverage and neointimal growth in a new glycyrrhizin acid (GA)-eluting stent. New Zealand White rabbits ( = 20) with atherosclerotic plaques were randomly divided into three groups based on implanted iliac artery stents: bare-metal stents (BMSs), rapamycin-eluting stents, and GA-eluting stents. After the intravascular ultrasound (IVUS) assessment at 28 days, the vessels were harvested for scanning electron microscopy (SEM) and histology. After 4 weeks of follow-up, the stent and external elastic lamina (EEL) areas were compared among the groups. The rapamycin- or GA-eluting stents significantly reduced the neointimal area compared with BMSs, though GA-eluting stents had the lowest reduction. There were more uncovered struts for rapamycin-eluting stents than those for GA-eluting stents and bare-metal stents. The endothelial nitric oxide synthase (eNOS) expression in GA-eluting stents was much higher than that in BMSs and rapamycin-eluting stents, even though the endothelial coverage between struts was equivalent between BMSs and GA-eluting stents. Moreover, GA-eluting stents markedly promoted re-endothelialization and improved arterial healing compared to rapamycin-eluting stents in a rabbit atherosclerotic model. In conclusion, the novel GA-coated stent used in this study inhibited intimal hyperplasia and promoted re-endothelialization.
大多数药物洗脱支架（DES）可抑制内膜增生，但会损害再内皮化。本研究旨在评估新型甘草次酸（GA）洗脱支架在体内的支架小梁覆盖率和新生内膜生长情况。将患有动脉粥样硬化斑块的20只新西兰白兔根据植入的髂动脉支架随机分为三组：裸金属支架（BMS）、雷帕霉素洗脱支架和GA洗脱支架。在28天时进行血管内超声（IVUS）评估后，采集血管进行扫描电子显微镜（SEM）检查和组织学检查。随访4周后，比较各组间支架和外弹力膜（EEL）面积。与BMS相比，雷帕霉素或GA洗脱支架显著减小了新生内膜面积，尽管GA洗脱支架减小的幅度最小。雷帕霉素洗脱支架未覆盖的支架小梁比GA洗脱支架和裸金属支架更多。GA洗脱支架中内皮型一氧化氮合酶（eNOS）的表达远高于BMS和雷帕霉素洗脱支架，尽管BMS和GA洗脱支架在支架小梁间的内皮覆盖率相当。此外，在兔动脉粥样硬化模型中，与雷帕霉素洗脱支架相比，GA洗脱支架显著促进了再内皮化并改善了动脉愈合。总之，本研究中使用的新型GA涂层支架抑制了内膜增生并促进了再内皮化。
