Ultralight 3D Graphene Oxide Aerogel Decorated with Pd-Fe Nanoparticles for the Simultaneous Detection of Eight Biomolecules.

In this proof-of-concept study, an ultralight graphene oxide aerogel (GOx-Aero) decorated with bimetallic palladium-iron nanoparticles (Pd-Fe) was synthesized and immobilized on a glassy carbon electrode (GCE) for electrochemical sensor applications. The main objective of this work was to develop a sensitive electrochemical sensor capable of simultaneously detecting eight biomolecules, including ascorbic acid (AA), dopamine (DA), uric acid (UA), 8-hydroxyguanine (8HG), guanine (G), adenine (A), thymine (T), and cytosine (C). To the best of our knowledge, this is the first time that an electrochemical sensor has been able to detect eight biomolecules simultaneously. The bimetallic GOx aerogel significantly enhanced the performance of the sensor by increasing the electroactive area, conductivity, and anodic peak current response. The sensor demonstrated sharp, well-defined, and continuous oxidation peaks for all eight analytes of interest and wide linear ranges of 5.0-1750, 0.25-100.0, 0.5-500.0, 0.5-375.0, 0.5-500.0, 0.5-500.0, 5.0-1500.0, and 5.0-1500.0 μM for AA, DA, UA, 8HG, G, A, T, and C, respectively. The prepared sensor also exhibited excellent stability, reproducibility, and sensitivity with a very low limit of detection (LOD) of 553.7, 1.8, 69.6, 43.2, 42.9, 72.3, 57.2, and 318.4 nM for AA, DA, UA, 8HG, G, A, T, and C, respectively. The Pd-Fe-GOx-Aero-GCE was also tested in various real samples such as artificial saliva, artificial cerebrospinal fluid (CSF), salmon sperm DNA, and genomic DNA from calf thymus, where it demonstrated good recovery values. Additionally, the novel developed sensor was used to monitor the interaction between the anticancer drug, cisplatin, which has well-described binding affinity with the G and A bases in DNA. Overall, Pd-Fe-GOx-Aero-GCE displayed an extremely promising platform not only for the simultaneous detection of eight biomolecules in complex biological matrices but also for DNA-drug interaction studies toward the development of electrochemical high-throughput drug screening assays, which is of great importance in the field.