College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China.
BGI-Shenzhen, Shenzhen, China.
Environ Health Perspect. 2023 Jun;131(6):67004. doi: 10.1289/EHP11874. Epub 2023 Jun 2.
Exposure to environmental pollutants, including benzo[a]pyrene (BaP), has been implicated in allergic diseases and intestinal microbiota homeostasis, but the environment-microbiota-immunity triangular relationship and to what extent BaP-induced remodeling of the gut microbiota contributes to intestinal allergic inflammation remain to be established.
We investigated the impact of BaP on intestinal allergic inflammation and examined the relationship between this effect and gut microbiota dysbiosis. We explored the potential ability of intestinal bacteria to degrade BaP and alleviate cytotoxicity as a detoxification strategy to counteract the effects of BaP exposure.
We combined microbiome shotgun metagenomics with animal histological and intestinal allergic inflammatory responses to assess the effects of BaP () in a 23-d toxicity test in antigen-induced allergic female mice. In addition, genome annotation, quantitative analysis of BaP, and cytotoxicity-tests using CaCo-2 cells were conducted to infer the role of intestinal bacteria in BaP detoxification.
BaP exposure impacted the taxonomic composition and the functional potential of the gut microbiota and aggravated antigen-induced intestinal allergic inflammatory responses. The level of inflammatory cytokines correlated with the abundance of specific bacterial taxa, including 28-4 and . We identified 614 bacteria harboring genes implicated in the degradation of BaP, and 4 of these bacterial strains were shown to significantly reduce the cytotoxicity of BaP to CaCo-2 cells .
Using allergic female mice as a model, we investigated the relationship between BaP, microbiota, and host immune reactions, highlighting the role of gut bacteria in BaP-aggravated allergic reactions. Our findings offer novel insight toward establishing the causal relationship between BaP exposure and the occurrence of allergic disorders. Identifying gut bacteria that degrade BaP may provide new strategies for ameliorating BaP cytotoxicity. https://doi.org/10.1289/EHP11874.
环境污染物（包括苯并[a]芘（BaP））的暴露与过敏性疾病和肠道微生物组稳态有关，但环境-微生物组-免疫三角关系以及 BaP 诱导的肠道微生物组重塑在多大程度上导致肠道过敏炎症仍有待确定。
我们研究了 BaP 对肠道过敏炎症的影响，并研究了这种影响与肠道微生物组失调的关系。我们探讨了肠道细菌降解 BaP 和减轻细胞毒性的潜在能力，作为一种解毒策略来抵消 BaP 暴露的影响。
我们结合宏基因组 shotgun 微生物组学和动物组织学以及肠道过敏炎症反应，评估了 BaP（）在抗原诱导的过敏雌性小鼠 23 天毒性试验中的作用。此外，还进行了基因组注释、BaP 的定量分析以及使用 CaCo-2 细胞进行的细胞毒性试验，以推断肠道细菌在 BaP 解毒中的作用。
BaP 暴露影响了肠道微生物组的分类组成和功能潜力，并加重了抗原诱导的肠道过敏炎症反应。炎症细胞因子的水平与特定细菌分类群的丰度相关，包括 28-4 和 。我们鉴定了 614 种携带参与 BaP 降解基因的细菌，其中 4 种细菌菌株显著降低了 BaP 对 CaCo-2 细胞的细胞毒性。
我们使用过敏雌性小鼠作为模型，研究了 BaP、微生物组和宿主免疫反应之间的关系，强调了肠道细菌在 BaP 加重过敏反应中的作用。我们的研究结果为确定 BaP 暴露与过敏疾病发生之间的因果关系提供了新的见解。鉴定降解 BaP 的肠道细菌可能为减轻 BaP 细胞毒性提供新的策略。https://doi.org/10.1289/EHP11874.
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