College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471000, China.
State Key Laboratory of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi 214122, China.
Food Chem. 2023 Oct 30;424:136478. doi: 10.1016/j.foodchem.2023.136478. Epub 2023 May 27.
Indicator replacement assay combining with fluorescence resonance energy transfer (FRET) effect has attractive performance in sensing small molecules, however, there wasn't application in pesticide molecule sensing reported so far. In this work, we prepared a nanocomplex (NCP), AuAgNCs-CD, through self-assembly of gold nanoclusters (AuNCs), silver nanoclusters (AgNCs) and carboxymethyl-β-cyclodextrin (CM-β-CD) by one-step method. The emission of AuNCs was significantly enhance. It was found that FRET between the AuAgNCs-CD and rhodamine B (RhB) existed after AuAgNCs-CD combined with RhB. And carbendazim (CBZ) could induce anti-FRET effect through competing with RhB and binding to AuAgNCs-CD. Thus, this phenomenon was utilized to develop a ratiometric fluorescent detection of CBA. This method was applied in food sample detection and reliable results were obtained. Due to high sensitivity, rapidness and good selectivity, this ratiometric fluorescent method was expected to hold high application potential in monitoring CBZ in foods.
基于指示剂替换分析结合荧光共振能量转移（FRET）效应的小分子传感具有吸引力，但到目前为止，尚未有在农药分子传感方面的应用。在这项工作中，我们通过一步法自组装金纳米簇（AuNCs）、银纳米簇（AgNCs）和羧甲基-β-环糊精（CM-β-CD）制备了纳米复合物（NCP）AuAgNCs-CD。AuNCs 的发射显著增强。发现 AuAgNCs-CD 与罗丹明 B（RhB）结合后存在 AuAgNCs-CD 与罗丹明 B 之间的 FRET。而且，多菌灵（CBZ）可以通过与 RhB 竞争并与 AuAgNCs-CD 结合来诱导反 FRET 效应。因此，这种现象被用于开发比率荧光检测 CBA。该方法已应用于食品样品检测，并获得了可靠的结果。由于高灵敏度、快速性和良好的选择性，这种比率荧光方法有望在监测食品中的 CBZ 方面具有很高的应用潜力。
