Ali S F, Buelke-Sam J, Slikker W
Toxicol Lett. 1986 Jun;31(3):195-201. doi: 10.1016/0378-4274(86)90126-8.
The effects of prenatal exposure to reserpine on [3H]spiroperidol binding in caudate nucleus at postnatal day (PND) 21 were investigated. Pregnant rats were dosed with 0, 0.375 or 0.750 mg/kg/day reserpine s.c. on days 12-15 of gestation. At PND 21, pups were killed and their brains were dissected and stored at -70 degrees C for analysis. Dopamine receptor binding was measured in membrane prepared from caudate nucleus of both sexes over a [3H]spiroperidol concentration range of 0.02 to 2.0 nM. Scatchard analysis revealed that the number of dopamine receptors (Bmax) in the membrane of female caudate nucleus was significantly decreased in a dose-dependent manner, while the dissociation constant (KD) was relatively unchanged. In male rats, neither Bmax nor KD was significantly reduced in either dose group. These results show that prenatal exposure to reserpine decreased the dopamine receptor number in caudate nucleus in a sex-dependent manner. This alteration may underly several sex-related behavioral changes that were previously found in offspring from identically treated dams.
研究了产前接触利血平对出生后第21天（PND 21）尾状核中[3H]螺哌啶醇结合的影响。在妊娠第12 - 15天，对怀孕大鼠皮下注射0、0.375或0.750 mg/kg/天的利血平。在PND 21时，处死幼崽，解剖其大脑并在-70℃下保存以供分析。在0.02至2.0 nM的[3H]螺哌啶醇浓度范围内，测量了两性尾状核制备的膜中的多巴胺受体结合。Scatchard分析显示，雌性尾状核膜中的多巴胺受体数量（Bmax）以剂量依赖性方式显著降低，而解离常数（KD）相对不变。在雄性大鼠中，两个剂量组的Bmax和KD均未显著降低。这些结果表明，产前接触利血平以性别依赖的方式降低了尾状核中的多巴胺受体数量。这种改变可能是先前在相同处理的母鼠后代中发现的几种与性别相关的行为变化的基础。
