Quik M, Spokes E G, Mackay A V, Bannister R
J Neurol Sci. 1979 Nov;43(3):429-37. doi: 10.1016/0022-510x(79)90021-2.
[3H]Spiperone binding was investigated in the caudate nucleus, substantia nigra (s. nigra) and frontal cortex of control subjects and of patients with Parkinson's disease and the Shy-Drager syndrome. Binding sites for [3H]spiperone were interpreted as dopamine receptors in caudate and s. nigra, and as 5-hydroxytryptamine (5-HT) receptors in frontal cortex. Scatchard analysis showed that the Bmax (maximal number of binding sites) in caudate was similar in the 3 groups, whereas in s. nigra the Bmax was reduced by approximately 60% in both Parkinsons disease and Shy-Drager syndrome. The dissociation constant (Kd) for [3H]spiperone binding in s. nigra was similar in the 3 groups. In caudate nucleus, the Kd was similar in control and Parkinson groups; however, there was a significant increase in the dissociation constant in the caudate nucleus from cases of Shy-Drager syndrome. No differences in binding characteristics were observed in the frontal cortex. These results are taken to reflect a loss of dopamine receptor sites in the s. nigra in both Parkinson's disease and Shy-Drager syndrome, and a reduced affinity of dopamine receptor sites in the caudate nucleus in Shy-Drager syndrome.
在对照组、帕金森病患者和夏伊-德雷格综合征患者的尾状核、黑质和额叶皮质中研究了[3H]螺哌隆结合情况。[3H]螺哌隆的结合位点在尾状核和黑质中被解释为多巴胺受体，在额叶皮质中被解释为5-羟色胺（5-HT）受体。斯卡查德分析表明，尾状核中的Bmax（最大结合位点数）在三组中相似，而在黑质中，帕金森病和夏伊-德雷格综合征患者的Bmax均降低了约60%。三组中黑质中[3H]螺哌隆结合的解离常数（Kd）相似。在尾状核中，对照组和帕金森病组的Kd相似；然而，夏伊-德雷格综合征患者尾状核中的解离常数显著增加。在额叶皮质中未观察到结合特征的差异。这些结果被认为反映了帕金森病和夏伊-德雷格综合征患者黑质中多巴胺受体位点的丧失，以及夏伊-德雷格综合征患者尾状核中多巴胺受体位点亲和力的降低。
