Lemeshko V V, Belostotskaia L I
Ukr Biokhim Zh (1978). 1986 May-Jun;58(3):87-90.
A method is described to obtain mitochondria whose matrix at the stage of organella washing is saturated by dimethyl sulphoxide in the concentration necessary to prevent the cryodamage of membranes. Such mitochondria, being frozen and stored in liquid nitrogen, retain the value of a respiratory control of the coefficient ADP/O and the respiratory chain activity for 1.0-1.5 and more months.
描述了一种获取线粒体的方法，在细胞器洗涤阶段，其基质被二甲基亚砜饱和，浓度为防止膜冷冻损伤所需的浓度。这种线粒体冷冻并保存在液氮中，可保持呼吸控制系数ADP/O的值和呼吸链活性1.0 - 1.5个月甚至更长时间。
