• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过多光谱和量热法研究喜树碱功能化银纳米粒子对人肺癌细胞的细胞毒性和 DNA 结合策略。

Exploring the cytotoxicity on human lung cancer cells and DNA binding stratagem of camptothecin functionalised silver nanoparticles through multi-spectroscopic, and calorimetric approach.

机构信息

School of Pure and Applied Physics, Mahatma Gandhi University, P.D Hills (P.O), Kottayam, Kerala, 686 560, India.

Bharata Mata College, Thrikkakara, Ernakulam, Kerala, 682032, India.

出版信息

Sci Rep. 2023 Jun 3;13(1):9045. doi: 10.1038/s41598-023-34997-w.

DOI:10.1038/s41598-023-34997-w
PMID:37270606
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10239481/
Abstract

The influence of nanoparticles inside the human body and their interactions with biological macromolecules need to be explored/studied prior to specific applications. The objective of this study is to find the potential of camptothecin functionalised silver nanoparticles (CMT-AgNPs) in biomedical applications. This article primarily investigates the binding stratagem of CMT-AgNPs with calf thymus DNA (ctDNA) through a series of spectroscopic and calorimetric methods and then analyses the anticancer activity and cytotoxicity of CMT-AgNPs. The nanoparticles were synthesized using a simple one pot method and characterized using UV-Visible, fourier transform infrared (FTIR) spectroscopy, X-ray diffraction and high-resolution transmission electron microscopy (HRTEM). The average size of CMT-AgNPs is 10 ± 2 nm. A group of experimental techniques such as UV-Visible spectrophotometry, fluorescence dye displacement assay, circular dichroism (CD) and viscosity analysis unravelled the typical groove binding mode of CMT-AgNPs with ctDNA. The CD measurement evidenced the minor conformational alterations of double helical structure of ctDNA in the presence of CMT-AgNPs. The information deduced from the isothermal titration calorimetry (ITC) experiment is that the binding was exothermic and spontaneous in nature. Moreover, all the thermodynamic binding parameters were extracted from the ITC data. The binding constants obtained from UV absorption experiments, fluorescence dye displacement studies and ITC were consistently in the order of 10 Mol. All these results validated the formation of CMT-AgNPs-ctDNA complex and the results unambiguously confirm the typical groove binding mode of CMT-AgNPs. An exhaustive in vitro MTT assay by CMT-AgNPs and CMT against A549, HT29, HeLa and L929 cell lines revealed the capability of CMT-AgNPs as a potential anticancer agent.

摘要

在将纳米粒子应用于特定领域之前，需要研究其在人体内的影响及其与生物大分子的相互作用。本研究的目的是探索喜树碱功能化银纳米粒子（CMT-AgNPs）在生物医学中的应用潜力。本文主要通过一系列光谱和量热法研究 CMT-AgNPs 与小牛胸腺 DNA（ctDNA）的结合策略，然后分析 CMT-AgNPs 的抗癌活性和细胞毒性。纳米粒子采用简单的一锅法合成，并通过紫外-可见分光光度法、傅里叶变换红外（FTIR）光谱、X 射线衍射和高分辨率透射电子显微镜（HRTEM）进行表征。CMT-AgNPs 的平均粒径为 10±2nm。一组实验技术，如紫外-可见分光光度法、荧光染料置换法、圆二色性（CD）和粘度分析，揭示了 CMT-AgNPs 与 ctDNA 的典型沟结合模式。CD 测量表明，在 CMT-AgNPs 的存在下，ctDNA 的双螺旋结构发生了轻微的构象变化。等温滴定量热法（ITC）实验得出的信息表明，结合是放热和自发的。此外，所有热力学结合参数都从 ITC 数据中提取出来。从紫外吸收实验、荧光染料置换研究和 ITC 获得的结合常数依次为 10 Mol。所有这些结果都验证了 CMT-AgNPs-ctDNA 复合物的形成，并且结果明确证实了 CMT-AgNPs 的典型沟结合模式。通过 CMT-AgNPs 和 CMT 对 A549、HT29、HeLa 和 L929 细胞系进行的详尽体外 MTT 测定表明，CMT-AgNPs 具有作为潜在抗癌剂的能力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/40ae7ca11520/41598_2023_34997_Fig26_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/5978a7d7a892/41598_2023_34997_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/aa438086014a/41598_2023_34997_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/e9395f08e178/41598_2023_34997_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/453eb67dfe22/41598_2023_34997_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/ebbb82f1e97d/41598_2023_34997_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/bf2617adb600/41598_2023_34997_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/86489063b596/41598_2023_34997_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/b43377a1bf11/41598_2023_34997_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/13025820dd00/41598_2023_34997_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/5198c33aa120/41598_2023_34997_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/5af45e518abd/41598_2023_34997_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/e02a9b3d39ca/41598_2023_34997_Fig12_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/06cb874df657/41598_2023_34997_Fig13_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/908a7c2eef28/41598_2023_34997_Fig14_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/14f03a2adb29/41598_2023_34997_Fig15_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/2885add9ce6e/41598_2023_34997_Fig16_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/b8dc3333da29/41598_2023_34997_Fig17_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/a5134e388aa2/41598_2023_34997_Fig18_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/4f5dc58f319e/41598_2023_34997_Fig19_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/0b0da6890767/41598_2023_34997_Fig20_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/9024f7908809/41598_2023_34997_Fig21_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/954478806a31/41598_2023_34997_Fig22_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/1cffe5fbe405/41598_2023_34997_Fig23_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/adfadd8a2041/41598_2023_34997_Fig24_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/d85b9ca2fa58/41598_2023_34997_Fig25_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/40ae7ca11520/41598_2023_34997_Fig26_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/5978a7d7a892/41598_2023_34997_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/aa438086014a/41598_2023_34997_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/e9395f08e178/41598_2023_34997_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/453eb67dfe22/41598_2023_34997_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/ebbb82f1e97d/41598_2023_34997_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/bf2617adb600/41598_2023_34997_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/86489063b596/41598_2023_34997_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/b43377a1bf11/41598_2023_34997_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/13025820dd00/41598_2023_34997_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/5198c33aa120/41598_2023_34997_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/5af45e518abd/41598_2023_34997_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/e02a9b3d39ca/41598_2023_34997_Fig12_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/06cb874df657/41598_2023_34997_Fig13_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/908a7c2eef28/41598_2023_34997_Fig14_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/14f03a2adb29/41598_2023_34997_Fig15_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/2885add9ce6e/41598_2023_34997_Fig16_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/b8dc3333da29/41598_2023_34997_Fig17_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/a5134e388aa2/41598_2023_34997_Fig18_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/4f5dc58f319e/41598_2023_34997_Fig19_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/0b0da6890767/41598_2023_34997_Fig20_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/9024f7908809/41598_2023_34997_Fig21_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/954478806a31/41598_2023_34997_Fig22_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/1cffe5fbe405/41598_2023_34997_Fig23_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/adfadd8a2041/41598_2023_34997_Fig24_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/d85b9ca2fa58/41598_2023_34997_Fig25_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/10239481/40ae7ca11520/41598_2023_34997_Fig26_HTML.jpg

相似文献

1
Exploring the cytotoxicity on human lung cancer cells and DNA binding stratagem of camptothecin functionalised silver nanoparticles through multi-spectroscopic, and calorimetric approach.通过多光谱和量热法研究喜树碱功能化银纳米粒子对人肺癌细胞的细胞毒性和 DNA 结合策略。
Sci Rep. 2023 Jun 3;13(1):9045. doi: 10.1038/s41598-023-34997-w.
2
A Minor Groove Binder with Significant Cytotoxicity on Human Lung Cancer Cells: The Potential of Hesperetin Functionalised Silver Nanoparticles.具有显著细胞毒性的小沟结合物对人肺癌细胞的影响：橙皮素功能化银纳米粒子的潜力。
J Fluoresc. 2024 Sep;34(5):2179-2196. doi: 10.1007/s10895-023-03409-7. Epub 2023 Sep 18.
3
Green synthesis of silver nanoparticles using Pongamia pinnata seed: Characterization, antibacterial property, and spectroscopic investigation of interaction with human serum albumin.利用水黄皮种子绿色合成银纳米颗粒：表征、抗菌性能及与人血清白蛋白相互作用的光谱研究
J Mol Recognit. 2017 Jan;30(1). doi: 10.1002/jmr.2565. Epub 2016 Sep 28.
4
Photophysical and thermodynamic evaluation on the in vitro and in silico binding profile of Camptothecin with DNA.喜树碱与 DNA 的体外和计算结合特性的光物理和热力学评价。
Biophys Chem. 2019 Mar;246:40-49. doi: 10.1016/j.bpc.2018.12.004. Epub 2018 Dec 24.
5
Ecofriendly synthesis of silver and gold nanoparticles by Euphrasia officinalis leaf extract and its biomedical applications.以贯叶金丝桃叶提取物为绿色合成试剂制备金银纳米粒子及其生物医学应用
Artif Cells Nanomed Biotechnol. 2018 Sep;46(6):1163-1170. doi: 10.1080/21691401.2017.1362417. Epub 2017 Aug 8.
6
Phytosynthesis of silver nanoparticles using Artemisia marschalliana Sprengel aerial part extract and assessment of their antioxidant, anticancer, and antibacterial properties.利用绵毛蒿地上部分提取物进行银纳米颗粒的植物合成及其抗氧化、抗癌和抗菌性能评估。
Int J Nanomedicine. 2016 Apr 29;11:1835-46. doi: 10.2147/IJN.S99882. eCollection 2016.
7
Photo-catalytic, anti-bacterial, and anti-cancer properties of phyto-mediated synthesis of silver nanoparticles from Artemisia tournefortiana Rchb extract.光催化、抗菌和抗癌特性：从阿尔泰紫菀提取物通过植物介导合成银纳米颗粒
J Photochem Photobiol B. 2017 Aug;173:640-649. doi: 10.1016/j.jphotobiol.2017.07.003. Epub 2017 Jul 6.
8
Phytosynthesis of Silver Nanoparticles Using Leaf Extract: Characterization and Evaluation of Antibacterial, Antioxidant, and Anticancer Activities.利用叶提取物合成银纳米粒子：抗菌、抗氧化和抗癌活性的表征和评价。
Int J Nanomedicine. 2021 Jan 6;16:15-29. doi: 10.2147/IJN.S265003. eCollection 2021.
9
Ultra-sonication-enhanced green synthesis of silver nanoparticles using leaf extract and their possible application.超声增强绿色合成银纳米粒子及其可能的应用。
Artif Cells Nanomed Biotechnol. 2022 Dec;50(1):177-187. doi: 10.1080/21691401.2022.2084100.
10
Green Synthesis of Silver Nanoparticles of Leaf Extract and their Cytotoxicity Activity against Neuroblastoma SHSY-5Y Cell Lines, Antimicrobial and Antioxidant Studies.叶提取物合成的银纳米粒子的绿色合成及其对神经母细胞瘤 SH-SY5Y 细胞系的细胞毒性活性、抗菌和抗氧化研究。
Recent Pat Nanotechnol. 2023;17(3):270-280. doi: 10.2174/1872210516666220520144453.

引用本文的文献

1
Canthaxanthin downregulates EGFR in NSCLC: network pharmacology, molecular docking, dynamics simulations, ADMET, and in-vitro analysis.角黄素下调非小细胞肺癌中的表皮生长因子受体：网络药理学、分子对接、动力学模拟、药物代谢动力学及体外分析
Mol Divers. 2025 Jun 14. doi: 10.1007/s11030-025-11246-y.
2
The effect of arsenic on mitochondrial fatty acid metabolism via inhibition of carnitine palmitoyltransferase 1B and choline kinase beta in C2C12 cells.砷通过抑制C2C12细胞中的肉碱棕榈酰转移酶1B和胆碱激酶β对线粒体脂肪酸代谢的影响。
PLoS One. 2025 May 29;20(5):e0320557. doi: 10.1371/journal.pone.0320557. eCollection 2025.
3
Pterostilbene as a potent ESR-1 in breast cancer therapy: insights from network pharmacology, molecular docking, dynamics simulations, ADMET, and in vitro analysis.

本文引用的文献

1
Antimicrobial, antioxidant, cytotoxicity and photocatalytic performance of Co doped ZnO nanoparticles biosynthesized using leaf extract.使用叶提取物生物合成的钴掺杂氧化锌纳米颗粒的抗菌、抗氧化、细胞毒性和光催化性能
J Environ Health Sci Eng. 2023 Feb 3;21(1):167-185. doi: 10.1007/s40201-023-00851-4. eCollection 2023 Jun.
2
Physicochemical, Morphological, and Cytotoxic Properties of Brazilian Jackfruit () Starch Scaffold Loaded with Silver Nanoparticles.负载银纳米颗粒的巴西菠萝蜜淀粉支架的物理化学、形态学及细胞毒性特性
J Funct Biomater. 2023 Mar 3;14(3):143. doi: 10.3390/jfb14030143.
3
Exploring the Interaction Between the Newly Designed Antitumor Zn(II) Complex and CT-DNA/BSA: Spectroscopic Methods, DFT Computational Analysis, and Docking Simulation.
紫檀芪作为乳腺癌治疗中一种有效的雌激素受体1（ESR-1）调节剂：来自网络药理学、分子对接、动力学模拟、药物代谢动力学/药物毒理学（ADMET）及体外分析的见解
Mol Divers. 2025 Feb 24. doi: 10.1007/s11030-025-11144-3.
4
Development, and characterization of lamotrigine-loaded bovine serum albumin nanoparticles using QbD approach.采用质量源于设计（QbD）方法开发并表征载有拉莫三嗪的牛血清白蛋白纳米粒
Drug Deliv. 2025 Dec;32(1):2460693. doi: 10.1080/10717544.2025.2460693. Epub 2025 Feb 3.
5
Rosmarinic Acid-Rich Extract-Derived Silver Nanoparticles: A Green Synthesis Approach for Multifunctional Biomedical Applications including Antibacterial, Antioxidant, and Anticancer Activities.迷迭香酸丰富提取物衍生的银纳米粒子：一种用于多功能生物医学应用的绿色合成方法，包括抗菌、抗氧化和抗癌活性。
Molecules. 2024 Mar 12;29(6):1250. doi: 10.3390/molecules29061250.
6
A Minor Groove Binder with Significant Cytotoxicity on Human Lung Cancer Cells: The Potential of Hesperetin Functionalised Silver Nanoparticles.具有显著细胞毒性的小沟结合物对人肺癌细胞的影响：橙皮素功能化银纳米粒子的潜力。
J Fluoresc. 2024 Sep;34(5):2179-2196. doi: 10.1007/s10895-023-03409-7. Epub 2023 Sep 18.
探究新型抗肿瘤 Zn(II) 配合物与 CT-DNA/BSA 的相互作用：光谱法、DFT 计算分析和对接模拟。
Appl Biochem Biotechnol. 2023 Oct;195(10):6276-6308. doi: 10.1007/s12010-023-04394-0. Epub 2023 Mar 1.
4
Green Synthesis of Silver Nanoparticles Using Salvadora persica and Caccinia macranthera Extracts: Cytotoxicity Analysis and Antimicrobial Activity Against Antibiotic-Resistant Bacteria.采用萨尔瓦多相思树和大苞鞘石斛提取物的银纳米粒子的绿色合成：细胞毒性分析和对抗抗生素耐药菌的抗菌活性。
Appl Biochem Biotechnol. 2023 Aug;195(8):5120-5135. doi: 10.1007/s12010-023-04407-y. Epub 2023 Feb 27.
5
Cyperotundone combined with adriamycin induces apoptosis in MCF-7 and MCF-7/ADR cancer cells by ROS generation and NRF2/ARE signaling pathway.环菠萝酮联合阿霉素通过 ROS 生成和 NRF2/ARE 信号通路诱导 MCF-7 和 MCF-7/ADR 癌细胞凋亡。
Sci Rep. 2023 Jan 25;13(1):1384. doi: 10.1038/s41598-022-26767-x.
6
Flow Cytometry Detection of Anthracycline-Treated Breast Cancer Cells: An Optimized Protocol.蒽环类药物处理的乳腺癌细胞的流式细胞术检测：优化方案
Curr Issues Mol Biol. 2022 Dec 28;45(1):164-174. doi: 10.3390/cimb45010013.
7
Design, synthesis, antitumor activity and ct-DNA binding study of photosensitive drugs based on porphyrin framework.基于卟啉骨架的光敏药物的设计、合成、抗肿瘤活性及 ct-DNA 结合研究。
Int J Biol Macromol. 2023 Mar 1;230:123147. doi: 10.1016/j.ijbiomac.2023.123147. Epub 2023 Jan 5.
8
The Preliminary Assessment of New Biomaterials Necessitates a Comparison of Direct and Indirect Cytotoxicity Methodological Approaches.新型生物材料的初步评估需要比较直接和间接细胞毒性的方法学途径。
Polymers (Basel). 2022 Oct 25;14(21):4522. doi: 10.3390/polym14214522.
9
Multi-spectroscopic, thermodynamic, and molecular docking/dynamic approaches for characterization of the binding interaction between calf thymus DNA and palbociclib.多光谱、热力学和分子对接/动力学方法研究帕博西尼与小牛胸腺 DNA 的结合相互作用。
Sci Rep. 2022 Aug 30;12(1):14723. doi: 10.1038/s41598-022-19015-9.
10
Design, synthesis, and biological evaluation of novel 7-substituted 10,11-methylenedioxy-camptothecin derivatives against drug-resistant small-cell lung cancer in vitro and in vivo.新型 7-取代 10,11-亚甲二氧基喜树碱衍生物的设计、合成及体外和体内抗耐药性小细胞肺癌活性评价。
Eur J Med Chem. 2022 Nov 5;241:114610. doi: 10.1016/j.ejmech.2022.114610. Epub 2022 Jul 20.