探讨表没食子儿茶素没食子酸酯和茶黄素在不同温度下的应激保护差异中的线粒体靶标。
Mitochondrial targets exploration of epigallocatechin gallate and theaflavin in regards to differences in stress protection under different temperatures.
机构信息
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, P. R. China; Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Ministry of Education, Wuhan, P. R. China.
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, P. R. China; Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Ministry of Education, Wuhan, P. R. China; Functional Food Engineering & Technology Research Center of Hubei Province, Wuhan, P. R. China.
出版信息
J Nutr Biochem. 2023 Sep;119:109400. doi: 10.1016/j.jnutbio.2023.109400. Epub 2023 Jun 3.
The study investigated the impacts of epigallocatechin gallate (EGCG) and theaflavin (TF1) on temperature tolerance of nematodes and explored targets on mitochondria. Survival rates, mitochondrial membrane potential (MMP) and ATP content of nematodes at different temperatures incubated with EGCG or TF1 were quantified. Thermogenesis and function of ex-vivo mitochondria were characterized. Targeted proteins of substances were identified via drug affinity responsive target stability (DARTS) and RT-qPCR. It turned out that EGCG and TF1 increased survival rates of nematodes under heat and cold stress, respectively. TF1 exhibited lower MMP of nematodes and more mitochondrial thermogenesis than EGCG for the cold-protection. Meanwhile, TF1 up-regulated gpi-1, pgk-1, acox-1.2, acox-1.3, and acaa-2 to compensate the energy loss due to the uncoupling and downregulation of sdha-1 and atp-1. EGCG up-regulated ctl-1, hsp-60 and enol-1 expression for the thermo-protection, as well as pgk-1, acox-1.3, and acaa-2 to compensate energy loss due to the downregulation of sdha-1.
该研究调查了表没食子儿茶素没食子酸酯（EGCG）和茶黄素（TF1）对线虫耐热性的影响，并探讨了在线粒体上的靶标。用 EGCG 或 TF1 孵育不同温度下的线虫的存活率、线粒体膜电位（MMP）和 ATP 含量进行了定量分析。对离体线粒体的产热和功能进行了表征。通过药物亲和反应靶标稳定性（DARTS）和 RT-qPCR 鉴定了物质的靶向蛋白。结果表明，EGCG 和 TF1 分别提高了线虫在热应激和冷应激下的存活率。TF1 对线虫 MMP 的影响较低，对冷保护的线粒体产热作用强于 EGCG。同时，TF1 上调了 gpi-1、pgk-1、acox-1.2、acox-1.3 和 acaa-2，以补偿由于解偶联和 sdha-1 和 atp-1 的下调而导致的能量损失。EGCG 上调了 ctl-1、hsp-60 和 enol-1 的表达以进行热保护，同时还上调了 pgk-1、acox-1.3 和 acaa-2，以补偿由于 sdha-1 的下调导致的能量损失。
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