Shah Murad-Ali, Kang Ju-Bin, Koh Phil-Ok
Department of Anatomy, College of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National University, 501 Jinjudaero, Jinju, 52828, South Korea.
Lab Anim Res. 2023 Jun 5;39(1):12. doi: 10.1186/s42826-023-00164-5.
Cerebral ischemia is a serious neurological disorder that can lead to high morbidity and mortality. Chlorogenic acid is a polyphenol compound with antioxidant that can regulate proteins in cerebral ischemia. Middle cerebral artery occlusion (MCAO) surgery was performed to induce ischemic brain injury and was maintained for 24 h. Chlorogenic acid (30 mg/kg) or vehicle was administrated into the peritoneal cavity 2 h after MCAO surgery. The cerebral cortical tissues were collected for further study and a proteomic approach was performed to identify the proteins changed by chlorogenic acid in the MCAO animals.
We found that chlorogenic acid alleviated in changes in adenosylhomocysteinase, glycerol-3-phosphate dehydrogenase, eukaryotic translation initiation factor 4A-II, apolipoprotein A-I, and mu-crystallin. These proteins were reduced in MCAO animals with vehicle, and these reductions were attenuated by chlorogenic acid treatment. The mitigation of this reduction by chlorogenic acid was confirmed by the reverse transcription PCR technique. These proteins are associated with energy metabolism, protein synthesis, inflammation, and physiological metabolism. They are involved in the neuroprotective effect of chlorogenic acid. These results showed that chlorogenic acid alleviates the neurological disorders caused by MCAO and regulates the expression of proteins involved in neuroprotection.
Therefore, our findings provide evidence that chlorogenic acid plays a neuroprotective role in stroke animal models by controlling specific proteins.
脑缺血是一种严重的神经疾病，可导致高发病率和死亡率。绿原酸是一种具有抗氧化作用的多酚化合物，可调节脑缺血中的蛋白质。进行大脑中动脉闭塞（MCAO）手术以诱导缺血性脑损伤，并维持24小时。在MCAO手术后2小时，将绿原酸（30mg/kg）或赋形剂注入腹腔。收集大脑皮质组织进行进一步研究，并采用蛋白质组学方法鉴定MCAO动物中因绿原酸而发生变化的蛋白质。
我们发现绿原酸减轻了腺苷同型半胱氨酸酶、甘油-3-磷酸脱氢酶、真核翻译起始因子4A-II、载脂蛋白A-I和μ-晶体蛋白的变化。在接受赋形剂的MCAO动物中，这些蛋白质减少，而绿原酸处理减弱了这些减少。通过逆转录PCR技术证实了绿原酸对这种减少的缓解作用。这些蛋白质与能量代谢、蛋白质合成、炎症和生理代谢有关。它们参与了绿原酸的神经保护作用。这些结果表明，绿原酸减轻了MCAO引起的神经疾病，并调节了参与神经保护的蛋白质的表达。
因此，我们的研究结果提供了证据，表明绿原酸通过控制特定蛋白质在中风动物模型中发挥神经保护作用。
