Graduate School of Biomedical Engineering, University of New South Wales, Sydney, New South Wales, Australia.
Australian Research Council Centre of Excellence for Nanoscale BioPhotonics, The University of Adelaide, Adelaide, South Australia, Australia.
J Biophotonics. 2023 Sep;16(9):e202300105. doi: 10.1002/jbio.202300105. Epub 2023 Jun 15.
Hyperspectral and multispectral imaging of cell and tissue autofluorescence is an emerging technology in which fluorescence imaging is applied to biological materials across multiple spectral channels. This produces a stack of images where each matched pixel contains information about the sample's spectral properties at that location. This allows precise collection of molecularly specific data from a broad range of native fluorophores. Importantly, complex information, directly reflective of biological status, is collected without staining and tissues can be characterised in situ, without biopsy. For oncology, this can spare the collection of biopsies from sensitive regions and enable accurate tumour mapping. For in vivo tumour analysis, the greatest focus has been on oral cancer, whereas for ex vivo assessment head-and-neck cancers along with colon cancer have been the most studied, followed by oral and eye cancer. This review details the scope and progress of research undertaken towards clinical translation in oncology.
细胞和组织自发荧光的高光谱和多光谱成像是一项新兴技术，其中荧光成像是在多个光谱通道上应用于生物材料。这会产生一叠图像，其中每个匹配的像素都包含有关该位置样品光谱特性的信息。这允许从广泛的天然荧光团中精确收集具有分子特异性的数据。重要的是，无需染色即可收集直接反映生物学状态的复杂信息，并且可以在不进行活检的情况下对组织进行原位表征。对于肿瘤学，可以避免从敏感区域采集活检，并能够进行准确的肿瘤绘图。对于体内肿瘤分析，最大的重点是口腔癌，而对于离体评估，头颈部癌症以及结肠癌是研究最多的，其次是口腔癌和眼癌。这篇综述详细介绍了在肿瘤学临床转化方面所进行的研究的范围和进展。
