Nemeth Daniel, Luqman Numana, Chen Loretta, Quan Ning
Stiles-Nicholson Brain Institute, College of Medicine, Florida Atlantic University, Boca Raton, Florida, United States.
Florida Atlantic University High School, Boca Raton, Florida, United States.
MicroPubl Biol. 2023 May 19;2023. doi: 10.17912/micropub.biology.000832. eCollection 2023.
The mouse is a widely used transgenic mouse model to conditionally express Cre recombinase in astrocytes of the central nervous system. Currently, no reports show whether the Cre recombinase activity, driven by the promoter, acts in cells outside of its intended astrocyte population. We crossed the mouse with a TdTomato reporter mouse line, ROSA26:CAG-LSL-TdTomato, to generate a fluorescent reporter for promoter activity. Gross anatomical observations reveal strong TdTomato expression in the spleen and exocrine glands-the salivary gland and the pancreas. We find TdTomato expression, a reporter of Cre activity, specifically targets serous cells in the parotid, submandibular, sublingual glands, and pancreas along with fibroblast-like cells within the submandibular lymph nodes and spleen. Our data indicate that the mouse model has unintended Cre recombinase activity in exocrine glands, which may influence biological and behavioral data.
该小鼠是一种广泛使用的转基因小鼠模型，用于在中枢神经系统的星形胶质细胞中条件性表达Cre重组酶。目前，尚无报告表明由该启动子驱动的Cre重组酶活性是否在其预期的星形胶质细胞群体之外的细胞中起作用。我们将该小鼠与TdTomato报告基因小鼠品系ROSA26:CAG-LSL-TdTomato杂交，以生成用于该启动子活性的荧光报告基因。大体解剖观察显示，脾脏和外分泌腺（唾液腺和胰腺）中有强烈的TdTomato表达。我们发现，作为Cre活性报告基因的TdTomato表达，特异性地靶向腮腺、颌下腺、舌下腺和胰腺中的浆液细胞，以及颌下淋巴结和脾脏内的成纤维细胞样细胞。我们的数据表明，该小鼠模型在外分泌腺中具有意外的Cre重组酶活性，这可能会影响生物学和行为数据。
