[Effect of cryopreservation on the acrosomal enzyme activity of the spermatozoa of rams].

Studied was the effect of cryoconservation on the activity of acrosine and acrosomal acid phosphatase of spermatozoa of rams stored in the form of pellets with the use of a diluent after Nagaze-Niva. It was found that the activity of acid phosphatase dropped by 35 per cent when stored in the course of 24 hours and 7 days. Acrosine was shown to be more stable at cryoconservation--its activity did not change under the same conditions of storing, however, it dropped by 30 per cent for 7 days. Admittedly, one of the reasons for the drop of the fertilizing capacity of ram spermatozoa after freezing was the drop of acrosomal enzyme activity. Most probably, the noxious factors were two--ultralow temperatures, and storage duration of spermatozoa.