Zhao Shuyan, Zeng Bo, Pei Rui, Fu Xiaoli, Zhu Meinan, Zhang Guangya, Jiang Wei
College of Chemical Engineering, Huaqiao University, Xiamen, Fujian, 361021, China.
School of Environmental Science and Engineering, Shandong University, Qingdao 266237, China.
Int J Bioprint. 2023 Feb 27;9(3):695. doi: 10.18063/ijb.695. eCollection 2023.
As an environmental pollutant, formaldehyde can cause serious harm to the human body. Among many degradation methods, formaldehyde dehydrogenase from (PFDH) exhibits broad potential because of its strong catalytic specificity and high degradation efficiency. However, the real application of PFDH in industry is limited by its instability and difficulties in recycling. In this work, the suitable printing conditions for immobilizing PFDH by three-dimensional (3D) printing technology were studied: the concentration of sodium alginate (SA) was 1.635 wt%, the concentration of CaCl was 7.4 wt%, the crosslinking time with CaCl was 8 min, and the temperature of the reaction was 31.5°C. 3D-printed PFDH/calcium alginate (CA) microspheres have 210% relative enzyme activity after seven repeated uses. Dried PFDH/CA particles were characterized by scanning electron microscope (SEM), Fourier transform infrared spectrometer (FT-IR), EDS elemental mapping, and thermogravimetric analysis (TGA) which proved that the enzyme was immobilized by the material. In addition, the recycling ability of 3D printing to immobilize different objects was explored and different shapes were designed by computer-aided design (CAD). In conclusion, 3D printing technology was applied to immobilize PFDH in this work, which provides a new idea to biodegrade formaldehyde in a green way.
作为一种环境污染物，甲醛会对人体造成严重危害。在众多降解方法中，来自[具体来源未给出]的甲醛脱氢酶（PFDH）因其强大的催化特异性和高降解效率而展现出广阔的潜力。然而，PFDH在工业上的实际应用受到其不稳定性和回收困难的限制。在这项工作中，研究了通过三维（3D）打印技术固定化PFDH的合适打印条件：海藻酸钠（SA）浓度为1.635 wt%，氯化钙（CaCl）浓度为7.4 wt%，与CaCl的交联时间为8分钟，反应温度为31.5°C。3D打印的PFDH/海藻酸钙（CA）微球在重复使用七次后具有210%的相对酶活性。通过扫描电子显微镜（SEM）、傅里叶变换红外光谱仪（FT-IR）、能谱元素映射和热重分析（TGA）对干燥的PFDH/CA颗粒进行了表征，证明酶被该材料固定化。此外，还探索了3D打印固定不同物体的回收能力，并通过计算机辅助设计（CAD）设计了不同形状。总之，这项工作将3D打印技术应用于固定化PFDH，为以绿色方式生物降解甲醛提供了新思路。
