Eager K B, Sawicki J A, Ricciardi R P
Virology. 1986 Jul 30;152(2):487-91. doi: 10.1016/0042-6822(86)90153-4.
Murine fibroblasts transformed by adenovirus 12 (Ad12) show reduced class I major histocompatibility complex (MHC) antigen synthesis and form tumors in syngeneic mice whereas those transformed by adenovirus 5 (Ad5) show no alteration in class I antigen synthesis and do not form tumors (K. B. Eager, J. Williams, D. Breiding, S. Pan, B. Knowles, E. Appella, and R. P. Ricciardi (1985) Proc. Natl. Acad. Sci. USA 82, 5525-5529). Nearly 1500 metabolically labeled polypeptides from the Ad5 and Ad12 transformed cell lines as well as polypeptides from a nontransformed murine line of the same haplotype were compared by two-dimensional gel electrophoresis. In addition to the reduction of the class I H-2 transplantation antigens seen in the Ad12-transformed lines, we detect few but reproducible polypeptide differences between the tumorigenic and nontumorigenic cell lines.
由腺病毒12（Ad12）转化的小鼠成纤维细胞显示出I类主要组织相容性复合体（MHC）抗原合成减少，并在同基因小鼠中形成肿瘤，而由腺病毒5（Ad5）转化的细胞在I类抗原合成方面没有变化，也不形成肿瘤（K. B. 伊格尔、J. 威廉姆斯、D. 布雷丁、S. 潘、B. 诺尔斯、E. 阿佩拉和R. P. 里恰尔迪（1985年）《美国国家科学院院刊》82，5525 - 5529）。通过二维凝胶电泳比较了来自Ad5和Ad12转化细胞系的近1500种经代谢标记的多肽，以及来自相同单倍型的未转化小鼠细胞系的多肽。除了在Ad12转化细胞系中观察到的I类H - 2移植抗原减少外，我们还检测到致瘤细胞系和非致瘤细胞系之间存在少量但可重复的多肽差异。
