一种用于测定α-白蛋白的双位点免疫放射分析方法。

A two-site immunoradiometric assay for the determination of alpha-albumin.

作者信息

Noppe M, Lowenthal A, Karcher D, Gheuens J

出版信息

J Immunol Methods. 1979 May 10;27(1):75-81. doi: 10.1016/0022-1759(79)90240-0.

Abstract

A two-site immunoradiometric assay for a brain specific protein (alpha-albumin or glial fibrillary acidic protein) is described which may be applied to other proteins. The assay variables studied are immunoadsorbent dilution, temperature, incubation times, reactivity of the labelled antibodies and effect of the washing. Each of these factors is optimalised, resulting in a sensitive, specific, reliable assay. The working range is 5--5000 ng/ml, the within-assay variance is 5%. No high dose hook effect is seen in this range. The method is applied for the measurement of alpha-albumin present in cerebrospinal fluid, nervous tissue extracts and cell cultures.

摘要

本文描述了一种用于检测脑特异性蛋白（α-白蛋白或胶质纤维酸性蛋白）的双位点免疫放射分析方法，该方法也可应用于其他蛋白质。所研究的分析变量包括免疫吸附剂稀释度、温度、孵育时间、标记抗体的反应性以及洗涤的影响。对这些因素进行了优化，从而得到了一种灵敏、特异、可靠的分析方法。其工作范围为5--5000 ng/ml，批内变异为5%。在此范围内未观察到高剂量钩效应。该方法用于检测脑脊液、神经组织提取物和细胞培养物中的α-白蛋白。