Khoulassa Siham, Khurshid Haris, Fokar Mohamed, Eliddrissy Hicham, Outeha Youssef, Elfadil Jamal, Essarioui Adil, Benlyas Mohamed, Mentag Rachid, Elmoualij Benaissa
National Institute of Agronomic Research (INRA), Errachidia, Morocco.
Moulay Ismail University (FSTE/UMI), Errachidia, Morocco.
Genet Mol Biol. 2023 Jun 5;46(2):e20220021. doi: 10.1590/1678-4685-GMB-2022-0021. eCollection 2023.
The genetic diversity between 23 Moroccan date palm cultivars collected from the National Palm Collection at the INRA (National Agricultural Research Institute) experimental field in Zagora was assessed using SSR markers that are specifically designed for date palm. Among the 16 tested SSR, 13 were successfully amplified, and were selected to carry out this study. 208 bands were amplified, ranging from 10 to 25 bands per cultivar with an average of 16 alleles per cultivar. The value of heterozygosity of the studied markers ranged from 0.11 to 0.30. The pairwise genetic distances between those cultivars ranged from 0.06 to 0.46. The hierarchical cluster analysis distributed the 23 genotypes into four different groups of one to ten cultivars.
利用专门为海枣设计的SSR标记,对从位于扎戈拉的国家农业研究所在因拉实验场的国家棕榈收集库中收集的23个摩洛哥海枣品种之间的遗传多样性进行了评估。在16个测试的SSR中,有13个成功扩增,并被选来开展这项研究。共扩增出208条带,每个品种的条带数在10至25条之间,每个品种平均有16个等位基因。所研究标记的杂合度值在0.11至0.30之间。这些品种之间的成对遗传距离在0.06至0.46之间。层次聚类分析将23个基因型分为一至十个品种的四个不同组。