Department of Human Biology, School of Nutrition and Translational Research in Metabolism (NUTRIM), Maastricht University Medical Centre+, the Netherlands.
Nuritas Ltd, Dublin, Ireland.
J Nutr. 2023 Jun;153(6):1718-1729. doi: 10.1016/j.tjnut.2023.01.014. Epub 2023 Jan 11.
Muscle mass and strength decrease during short periods of immobilization and slowly recover during remobilization. Recent artificial intelligence applications have identified peptides that appear to possess anabolic properties in in vitro assays and murine models.
This study aimed to compare the impact of Vicia faba peptide network compared with milk protein supplementation on muscle mass and strength loss during limb immobilization and regain during remobilization.
Thirty young (24 ± 5 y) men were subjected to 7 d of one-legged knee immobilization followed by 14 d of ambulant recovery. Participants were randomly allocated to ingest either 10 g of the Vicia faba peptide network (NPN_1; n = 15) or an isonitrogenous control (milk protein concentrate; MPC; n = 15) twice daily throughout the study. Single-slice computed tomography scans were performed to assess quadriceps cross-sectional area (CSA). Deuterium oxide ingestion and muscle biopsy sampling were applied to measure myofibrillar protein synthesis rates.
Leg immobilization decreased quadriceps CSA (primary outcome) from 81.9 ± 10.6 to 76.5 ± 9.2 cm and from 74.8 ± 10.6 to 71.5 ± 9.8 cm in the NPN_1 and MPC groups, respectively (P < 0.001). Remobilization partially recovered quadriceps CSA (77.3 ± 9.3 and 72.6 ± 10.0 cm, respectively; P = 0.009), with no differences between the groups (P > 0.05). During immobilization, myofibrillar protein synthesis rates (secondary outcome) were lower in the immobilized leg (1.07% ± 0.24% and 1.10% ± 0.24%/d, respectively) than in the non-immobilized leg (1.55% ± 0.27% and 1.52% ± 0.20%/d, respectively; P < 0.001), with no differences between the groups (P > 0.05). During remobilization, myofibrillar protein synthesis rates in the immobilized leg were greater with NPN_1 than those with MPC (1.53% ± 0.38% vs. 1.23% ± 0.36%/d, respectively; P = 0.027).
NPN_1 supplementation does not differ from milk protein in modulating the loss of muscle size during short-term immobilization and the regain during remobilization in young men. NPN_1 supplementation does not differ from milk protein supplementation in modulating the myofibrillar protein synthesis rates during immobilization but further increases myofibrillar protein synthesis rates during remobilization.
在短期固定期间，肌肉质量和力量会下降，而在重新活动期间则会缓慢恢复。最近的人工智能应用已经确定了一些肽，这些肽在体外试验和小鼠模型中似乎具有合成代谢特性。
本研究旨在比较 Vicia faba 肽网络与牛奶蛋白补充剂对肢体固定期间肌肉质量和力量损失以及重新活动期间恢复的影响。
30 名年轻（24 ± 5 岁）男性进行了 7 天的单腿膝关节固定，随后进行了 14 天的步行恢复。参与者被随机分配每天两次摄入 10 克 Vicia faba 肽网络（NPN_1；n = 15）或等氮对照（牛奶蛋白浓缩物；MPC；n = 15）。进行单次切片计算机断层扫描以评估股四头肌横截面积（CSA）。氘氧化物摄入和肌肉活检采样用于测量肌原纤维蛋白合成率。
腿部固定使股四头肌 CSA（主要结果）从 81.9 ± 10.6 减少到 76.5 ± 9.2 cm 和从 74.8 ± 10.6 减少到 71.5 ± 9.8 cm，分别在 NPN_1 和 MPC 组（P < 0.001）。重新活动部分恢复了股四头肌 CSA（分别为 77.3 ± 9.3 和 72.6 ± 10.0 cm；P = 0.009），但两组之间无差异（P > 0.05）。在固定期间，固定腿的肌原纤维蛋白合成率（次要结果）低于非固定腿（分别为 1.07% ± 0.24%和 1.10% ± 0.24%/d）（P < 0.001），但两组之间无差异（P > 0.05）。在重新活动期间，与 MPC 相比，NPN_1 使固定腿的肌原纤维蛋白合成率更高（分别为 1.53% ± 0.38%和 1.23% ± 0.36%/d；P = 0.027）。
在年轻男性中，NPN_1 补充剂在调节短期固定期间肌肉大小的损失和重新活动期间的恢复方面与牛奶蛋白没有区别。NPN_1 补充剂在调节固定期间的肌原纤维蛋白合成率方面与牛奶蛋白补充剂没有区别，但在重新活动期间进一步增加了肌原纤维蛋白合成率。
