The Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, National Institute for Biotechnology in the Negev, The Ben-Gurion University of the Negev, 84105, Beer Sheva, Israel.
Stem Cell Res Ther. 2023 Jun 6;14(1):152. doi: 10.1186/s13287-023-03377-6.
Adult hematopoietic stem cells (HSCs) in the bone marrow (BM) are quiescent. Following perturbations, such as blood loss or infection, HSCs may undergo activation. Surprisingly, little is known about the earliest stages of HSCs activation. We utilize surface markers of HSCs activation, CD69 and CD317, revealing a response as early as 2 h after stimulation. The dynamic expression of HSCs activation markers varies between viral-like (poly-Inosinic-poly-Cytidylic) or bacterial-like (Lipopolysaccharide) immune stimuli. We further quantify dose response, revealing a low threshold, and similar sensitivity of HSCs and progenitors in the BM. Finally, we find a positive correlation between the expression of surface activation markers and early exit from quiescence. Our data show that the response of adult stem cells to immune stimulation is rapid and sensitive, rapidly leading HSCs out of quiescence.
骨髓中的成人造血干细胞（HSCs）处于静止状态。在受到诸如失血或感染等干扰后，HSCs 可能会被激活。令人惊讶的是，人们对 HSCs 激活的最早阶段知之甚少。我们利用 HSCs 激活的表面标志物 CD69 和 CD317 发现，刺激后仅 2 小时就出现了反应。病毒样（多聚肌苷酸-多聚胞苷酸）或细菌样（脂多糖）免疫刺激物的 HSCs 激活标志物的动态表达有所不同。我们进一步量化了剂量反应，发现 HSCs 和骨髓中的祖细胞具有低阈值和相似的敏感性。最后，我们发现表面激活标志物的表达与早期退出静止状态之间存在正相关。我们的数据表明，成年干细胞对免疫刺激的反应是迅速而敏感的，这会导致 HSCs 迅速脱离静止状态。
