Kishimoto Takeo, Kubota Junko, Kanatani Haruo
Laboratory of Reproductive Biology, National Institute for Basic Biology, Myodaiji, Okazaki 444.
Laboratory of Physiology, Ocean Research Institute, University of Tokyo, Nakano, Tokyo 164, Japan.
Dev Growth Differ. 1977;19(4):283-288. doi: 10.1111/j.1440-169X.1977.00283.x.
In starfish, cytoplasm taken from maturing oocytes is capable of inducing breakdown of the germinal vesicle and subsequent maturation when injected into immature oocytes. The cytoplasmic factor has been designated as "maturation-promoting factor" (MPF). Ooplasm was stratified by centrifugation of maturing oocytes in density-graded Ficoll-seawater, without disrupting the cell. Three strata were distinguished beginning with the centripetal side: oil cap, hyaline layer and yellow layer. MPF activity was shown to be localized in the hyaline layer. Electron microscopic observation revealed that the hyaline layer contains Golgi complexes, many lucent vesicles and multi-vesicular bodies as distinct organelles, but seldom contains such inclusions as the lipid droplets forming the oil cap, mitochondria, yolk and pigment granules contained in the yellow layer. Based on these observations, a possible cytoplasmic component with MPF activity is discussed.
在海星中，取自成熟卵母细胞的细胞质在注入未成熟卵母细胞时，能够诱导生发泡破裂并随后成熟。这种细胞质因子被命名为“成熟促进因子”（MPF）。通过在密度梯度的菲可-海水中对成熟卵母细胞进行离心分层，而不破坏细胞。从向心侧开始区分出三层：油帽、透明层和黄色层。结果表明MPF活性定位于透明层。电子显微镜观察显示，透明层含有高尔基体复合物、许多透明小泡和多囊体等独特的细胞器，但很少含有诸如形成油帽的脂滴、黄色层中含有的线粒体、卵黄和色素颗粒等内含物。基于这些观察结果，讨论了一种可能具有MPF活性的细胞质成分。
