Shinagawa Atsunori, Konno Seiko, Yoshimoto Yasuaki, Hiramoto Yukio
Department of Biology, Faculty of Science, Yamagata University, Yamagata 990.
Developmental and Reproductive Biology Center, Shironoshi 5-34-5, Yamagata 990.
Dev Growth Differ. 1989 Jun;31(3):249-255. doi: 10.1111/j.1440-169X.1989.00249.x.
The initiation site of surface contraction waves (SCWs) was examined in fertilized, parthenogenetically activated and enucleated Xenopus eggs after either rotation through 90° off the vertical axis or injection of colchicine. In enucleated eggs, SCWs always started from a top site of the egg under all conditions examined. In fertilized or activated eggs, SCWs started, depending on the experimental conditions, from either the sperm entry point, the animal pole region located sideward or the top site of the egg. Histological examinations of fertilized and activated eggs revealed that the nucleus was in most cases positioned close to the initiation site of SCWs under various experimental conditions. It is suggested from these results that the egg cytoplasm has an intrinsic capability of causing the surface to generate SCWs, and that the nucleus is generally involved in localizing the initiation site of SCWs in fertilized or activated Xenopus eggs. A possible mechanism for localizing the initiation site of SCWs in Xenopus eggs is proposed.
在经垂直轴旋转90°或注射秋水仙碱处理的受精、孤雌激活和去核非洲爪蟾卵中，研究了表面收缩波（SCWs）的起始位点。在去核卵中，在所有检测条件下，SCWs总是从卵的顶部位点起始。在受精或激活的卵中，根据实验条件，SCWs起始于精子入卵点、侧向的动物极区域或卵的顶部位点。对受精和激活卵的组织学检查显示，在各种实验条件下，细胞核在大多数情况下位于SCWs起始位点附近。从这些结果推测，卵细胞质具有使表面产生SCWs的内在能力，并且细胞核通常参与在受精或激活的非洲爪蟾卵中定位SCWs的起始位点。提出了一种在非洲爪蟾卵中定位SCWs起始位点的可能机制。
