Nilsson N O, Belfrage P
J Lipid Res. 1979 May;20(4):557-60.
A methods for direct and continuous monitoring of free fatty acid release in adipocyte suspensions is described. Using a pH-stat apparatus the protons from the released free fatty acids are continuously titrated and the accumulated amount of OH- added is monitored on a recorder against time, the slope thus indicating the rate of free fatty acid release. Sinc pH is kept constant, an incubation medium with a low buffering capacity can be used, which gives the method a high sensitivity. Under the conditions described, free fatty acid release from 5% of maximal norepinephrine stumulation of rat adipocytes can be accurately measured and the kinetics can be followed over extended periods of time.
本文描述了一种直接连续监测脂肪细胞悬液中游离脂肪酸释放的方法。使用pH计装置，释放出的游离脂肪酸中的质子被连续滴定，记录器上记录添加的OH-累积量随时间的变化，其斜率即表示游离脂肪酸的释放速率。由于pH保持恒定，因此可使用缓冲能力低的孵育培养基，这使得该方法具有高灵敏度。在所描述的条件下，可准确测量大鼠脂肪细胞最大去甲肾上腺素刺激量的5%所释放的游离脂肪酸，并可长时间跟踪其动力学。
