Meisner H, Tenney K
J Lipid Res. 1977 Nov;18(6):774-6.
A convenient method of measuring initial rates of free fatty acid efflux from isolated adipocytes during triglyceride breakdown by hormone-sensitive lipase is described. The procedure is based on the dissociation of protons from carboxyl groups of free fatty acids. A recording pH meter is used to monitor H+ concentration in the medium continuously as an index of free fatty acid release. A stoichiometric relationship was demonstrated between proton release and extracellular free fatty acid concentration as determined by the 63Ni radioassay method of Ho (1970. Anal. Biochem. 36: 105-113). An acid pH (6.8) caused a reduction in proton release, which was immediately and completely reversed by raising the pH to 7.4.
本文描述了一种简便的方法，用于测量激素敏感性脂肪酶催化甘油三酯分解过程中，分离的脂肪细胞中游离脂肪酸流出的初始速率。该方法基于游离脂肪酸羧基中质子的解离。使用记录式pH计连续监测培养基中的H⁺浓度，作为游离脂肪酸释放的指标。通过Ho（1970年，《分析生物化学》36卷：105 - 113页）的⁶³Ni放射分析方法确定，质子释放与细胞外游离脂肪酸浓度之间存在化学计量关系。酸性pH（6.8）会导致质子释放减少，将pH提高到7.4可立即完全逆转这种情况。
