Urase Koko, Yasugi Sadao
Zoological Institute, Faculty of Science, University of Tokyo, Hongo, Bunkyo, Tokyo 113, Japan.
Department of Biology, Faculty of Science, Tokyo Metropolitan University, 1-1, Minamiosawa, Hachioji, Tokyo 192-03, Japan.
Dev Growth Differ. 1993 Feb;35(1):33-40. doi: 10.1111/j.1440-169X.1993.00033.x.
Epithelial-mesenchymal interaction plays an important role in the differentiation of digestive tract. However, the factors of these mesenchymes involved in induction of the epithelial differentiation of each organs are still unknown. In the present study, we made reconstituted mesenchymal cell aggregates by mixing proventricular mesenchymal cells with other mesenchymal cells, recombined the reconstituted mesenchyme with gizzard epithelium, and observed the differentiation of the gizzard epithelium in the explants with special attention to the appearance of embryonic chicken pepsinogen, one of the molecular marker of the proventricular epithelial cells, in the gizzard epithelium. The results showed that the proventricular mesenchymal cells induce gland formation and pepsinogen in the gizzard epithelium and that the esophageal and gizzard mesenchymal cells have the inhibitory influence on the differentiation of epithelia toward proventricular epithelium. The cells from small-intestinal, lung and dorsal dermal mesenchyme have no such effect. Based on the results obtained so far, a hypothesis was presented to explain the mechanism regulating the differentiation of the epithelium in the digestive tract in the chicken embryo.
上皮-间充质相互作用在消化道分化中起重要作用。然而，参与诱导各器官上皮分化的这些间充质的因素仍不清楚。在本研究中，我们通过将腺胃间充质细胞与其他间充质细胞混合制备重组间充质细胞聚集体，将重组间充质与肌胃上皮重组，并观察外植体中肌胃上皮的分化，特别关注腺胃上皮细胞的分子标志物之一——胚胎鸡胃蛋白酶原在肌胃上皮中的出现情况。结果表明，腺胃间充质细胞诱导肌胃上皮形成腺体和胃蛋白酶原，而食管和肌胃间充质细胞对上皮向腺胃上皮的分化具有抑制作用。来自小肠、肺和背部真皮间充质的细胞没有这种作用。基于目前获得的结果，提出了一个假说来解释鸡胚消化道上皮分化的调控机制。
