Ishizaki Hironori, Mizoguchi Akira, Fujishita Mariko, Suzuki Atsushi, Moriya Ikuo, O'Oka Hisayoshi, Kataoka Hiroshi, Isogai Akira, Nagasawa Hiromichi, Tamura Saburo, Suzuki Akinori
Biological Institute, Faculty of Science, Nagoaya University, Chikusa-ku, Nagoya 464, Japan.
Department of Agricultural Chemistry, The University of Tokyo, Bunkyo-ku, Tokyo 113, Japan.
Dev Growth Differ. 1983;25(6):593-600. doi: 10.1111/j.1440-169X.1983.00593.x.
Crude extracts of Bombyx mori brains can provoke adult development when injected into brain-removed dormant pupae of Bombyx mori and Samia Cynthia ricini. From this fact the prothoracicotropic hormone (PTTH) of Bombyx has long been thought to be species-nonspecifically active on Samia. Chemical fractionation of Bombyx brain or head extracts by fractional precipitation with acetone, Sephadex G-50 gel-filtration, and DEAE-Sepharose CL-6B chromatography, however, separated the fractions which activated Bombyx brainless pupae from those which activated Samia. Those results reveal the existence of two species-specific PTTHs.
将家蚕脑的粗提物注射到家蚕和蓖麻蚕去除脑的休眠蛹中，可促使其成虫发育。基于这一事实，长期以来人们一直认为家蚕的促前胸腺激素（PTTH）对蓖麻蚕具有非物种特异性活性。然而，通过用丙酮分级沉淀、Sephadex G - 50凝胶过滤和DEAE - Sepharose CL - 6B色谱法对家蚕脑或头部提取物进行化学分级分离，可将激活家蚕无脑蛹的组分与激活蓖麻蚕的组分分开。这些结果揭示了两种物种特异性PTTH的存在。
