Fujiwara Akiko, Hino Akiya, Hiruma Takaaki, Yasumasu Ikuo
Department of Biology, School of Education, Waseda University, 1-6-1, Nishiwaseda, Shinjuku-ku, Tokyo, 160, Japan.
Dev Growth Differ. 1982;24(2):145-154. doi: 10.1111/j.1440-169X.1982.00145.x.
In spermatozoa of all examined sea urchins, the respiration was inhibited and their motility was lowered by the glutaraldehyde-fixed eggs. The respiration of the fixed-egg-reacted spermatozoa was stimulated by 2, 4 dinitrophenol in Clypeaster japonicus, Hemicentrotus pulcherrimus and pseudocentrotus depressus but was not in Anthocidaris crassispina and Toxopneustes pileolus. Ratio of ADP to ATP was markedly lower in the reacted spermatozoa of the former species than in those of Anthocidaris. The low respiratory rate in the former species probably results from ADP control but does not in the latter species. Tetramethyl-p-phenylenediamine enhanced the respiratory rate in the reacted spermatozoa of the latter species to almost the same rate as in the intact spermatozoa, but elevated slightly in the former species. The inhibition of electron transport in mitochondrial respiratory chain is probably predominant in the latter species. In the former species, the slight inhibition of electron transport does not seem to result in a failure of ADP phosphorylation, and hence the stop of movement probably causes a shortage of ADP. Carnitine, which made the reacted spermatozoa of all species motile, enhanced the respiratory rate only in those of the former species.
在所有被检测的海胆精子中，戊二醛固定的卵子会抑制精子的呼吸并降低其活力。在日本海胆、马粪海胆和凹裂星海胆中，2,4-二硝基苯酚能刺激与固定卵子反应后的精子的呼吸，但在厚刺海胆和毒棘海胆中则不能。前几种海胆中与卵子反应后的精子的ADP与ATP的比率明显低于厚刺海胆中反应后的精子。前几种海胆中较低的呼吸速率可能是由ADP控制导致的，但后一种海胆并非如此。四甲基对苯二胺能将后一种海胆中与卵子反应后的精子的呼吸速率提高到几乎与完整精子相同的水平，但在前几种海胆中只能使其略有提高。后一种海胆中，线粒体呼吸链中的电子传递抑制可能占主导地位。在前几种海胆中，电子传递的轻微抑制似乎并未导致ADP磷酸化失败，因此运动停止可能导致ADP短缺。肉碱能使所有物种中与卵子反应后的精子恢复运动能力，且仅能提高前几种海胆中精子的呼吸速率。
