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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Suzuki N, Kajiura H, Nomura K, Garbers D L, Yoshino K, Kurita M, Tanaka H, Yamaguchi M

Noto Marine Laboratory, Kanazawa University, Ishikawa, Japan.

Comp Biochem Physiol B. 1988;89(4):687-93. doi: 10.1016/0305-0491(88)90309-4.

Fourteen peptides were isolated from the egg jelly of sea urchins, Pseudocentrotus depressus, Strongylocentrotus purpuratus, Hemicentrotus pulcherrimus and Anthocidaris crassispina and their amino acid sequences were determined. 2. The peptides stimulated H. pulcherrimus sperm respiration one half-maximally at about 8-60 pM. 3. Addition of speract to intact spermatozoa of P. depressus, H. pulcherrimus and A. crassispina resulted in the appearance of a newly stained protein (Mr 128,000 for P. depressus, Mr 128,000 for H. pulcherrimus and Mr 131,000 for A. crassispina) on sodium dodecyl sulfate-polyacrylamide gels.

从虾夷马粪海胆、紫海胆、光棘球海胆和粗刺海胆的卵胶中分离出14种肽，并测定了它们的氨基酸序列。2. 这些肽在约8 - 60皮摩尔时能使光棘球海胆精子呼吸达到最大刺激强度的一半。3. 向虾夷马粪海胆、光棘球海胆和粗刺海胆的完整精子中添加精子活化肽，在十二烷基硫酸钠 - 聚丙烯酰胺凝胶上会出现一种新的染色蛋白（虾夷马粪海胆的分子量为128,000，光棘球海胆的分子量为128,000，粗刺海胆的分子量为131,000）。

Suzuki N, Kajiura H, Nomura K, Garbers D L, Yoshino K, Kurita M, Tanaka H, Yamaguchi M

Noto Marine Laboratory, Kanazawa University, Ishikawa, Japan.

Comp Biochem Physiol B. 1988;89(4):687-93. doi: 10.1016/0305-0491(88)90309-4.

Fourteen peptides were isolated from the egg jelly of sea urchins, Pseudocentrotus depressus, Strongylocentrotus purpuratus, Hemicentrotus pulcherrimus and Anthocidaris crassispina and their amino acid sequences were determined. 2. The peptides stimulated H. pulcherrimus sperm respiration one half-maximally at about 8-60 pM. 3. Addition of speract to intact spermatozoa of P. depressus, H. pulcherrimus and A. crassispina resulted in the appearance of a newly stained protein (Mr 128,000 for P. depressus, Mr 128,000 for H. pulcherrimus and Mr 131,000 for A. crassispina) on sodium dodecyl sulfate-polyacrylamide gels.

从虾夷马粪海胆、紫海胆、光棘球海胆和粗刺海胆的卵胶中分离出14种肽，并测定了它们的氨基酸序列。2. 这些肽在约8 - 60皮摩尔时能使光棘球海胆精子呼吸达到最大刺激强度的一半。3. 向虾夷马粪海胆、光棘球海胆和粗刺海胆的完整精子中添加精子活化肽，在十二烷基硫酸钠 - 聚丙烯酰胺凝胶上会出现一种新的染色蛋白（虾夷马粪海胆的分子量为128,000，光棘球海胆的分子量为128,000，粗刺海胆的分子量为131,000）。