Tanimoto Satomi, Morisawa Masaaki
Department of Biology, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi 274, Japan.
Ocean Research Institute, University of Tokyo, 1-15-1 Minamidai, Nakano, Tokyo 164, Japan.
Dev Growth Differ. 1988 Apr;30(2):117-124. doi: 10.1111/j.1440-169X.1988.00117.x.
It is well known that the motility of spermatozoa in rainbow trout is suppressed by K . We showed here that although trout sperm are completely immotile in medium containing 5 mM K , motility was initiated by the subsequent addition of several mM Ca , suggesting that both K and Ca are related to the process of the initiation of sperm motility. It was further found that K channel blockers tetraethylammonium, nonyltriethylammonium, Ba and Cs , as well as the Ca channel blocker verapamil, inhibited the initiation of sperm motility at doses at which these reagents inhibit chnnel-related functions in other cells. However, Na channel blocker, tetrodotoxin and anion channel blocker 4, 4-diisothiocyatatostilbene-2, 2'-disulfonic acid inhibited the motility only at extremely high doses. These results suggest that transport of K and Ca through ion channels at the plasma membrane of spermatozoa is the first event that triggers the initiation of sperm motility in rainbow trout.
众所周知，虹鳟鱼精子的活力会受到钾离子（K⁺）的抑制。我们在此表明，尽管在含有5 mM钾离子的培养基中，鳟鱼精子完全不游动，但随后添加几毫摩尔的钙离子（Ca²⁺）会引发精子运动，这表明钾离子和钙离子都与精子运动起始过程有关。进一步发现，钾离子通道阻滞剂四乙铵、壬基三乙铵、钡离子（Ba²⁺）和铯离子（Cs⁺），以及钙离子通道阻滞剂维拉帕米，在这些试剂抑制其他细胞中通道相关功能的剂量下，会抑制精子运动的起始。然而，钠离子通道阻滞剂河豚毒素和阴离子通道阻滞剂4,4'-二异硫氰酸根合芪-2,2'-二磺酸仅在极高剂量下才会抑制精子运动。这些结果表明，钾离子和钙离子通过精子质膜上的离子通道转运是触发虹鳟鱼精子运动起始的首要事件。
