Morio Takahiro, Ozaki Toshinori, Takeuchi Ikuo, Tasaka Masao
Division of Developmental Biology, National Institute for Basic Biology, 38 Nishigonaka, Myodaijicho, Okazaki 444, Japan.
Dev Growth Differ. 1991 Aug;33(4):293-298. doi: 10.1111/j.1440-169X.1991.00293.x.
We have previously isolated several cell-type-enriched mRNAs of Dictyostelium discoideum. Since the temporal pattern of appearance and cell-type-enrichment of these RNAs were examined only by determining their accumulation, it was unclear whether their accumulation is regulated at the transcription level or the post-transcriptional level. To distinguish between these two possibilities, we examined the temporal and cell-type-enriched transcription of several of these genes by nuclear run-on assay. The results suggest that some genes are controlled in both temporal accumulation and cell-type-enrichment at the transcriptional level, but post-transcriptional regulation is also important for regulating cell-type enrichment in the case of some other genes.
