花粉发育过程中5S核糖体RNA的合成

THE SYNTHESIS OF 5S RIBOSOMAL RNA DURING POLLEN DEVELOPMENT.

作者信息

Peddada Lorraine B, Mascarenhas Joseph P

机构信息

Department of Biological Sciences, State University of New York at Albany, Albany, N.Y. 12222.

出版信息

Dev Growth Differ. 1975;17(1):1-8. doi: 10.1111/j.1440-169X.1975.00001.x.

DOI:10.1111/j.1440-169X.1975.00001.x

PMID:37281964

Abstract

Tradescantia paludosa 5S ribosomal RNA (rRNA) has been characterized with respect to its base composition and relative electrophoretic mobility in comparison with that of E. coli. The period of 5S rRNA synthesis during pollen grain development was determined by pulse labeling the RNA synthesized during a 24 hr period of development with P and then chasing in cold medium until pollen maturity. The period of highest 5S rRNA synthesis was found to occur prior to microspore mitosis. During and following mitosis over a period of 4 days there was a sharp decrease in the amount of 5S RNA synthesized and during the last 48 hr of pollen maturation, no 5S rRNA was synthesized.

摘要

与大肠杆菌的5S核糖体RNA（rRNA）相比，对紫露草5S rRNA的碱基组成和相对电泳迁移率进行了表征。通过用³²P脉冲标记在24小时发育期间合成的RNA，然后在冷培养基中追踪直至花粉成熟，来确定花粉粒发育过程中5S rRNA的合成时期。发现5S rRNA合成最高的时期发生在小孢子有丝分裂之前。在有丝分裂期间及之后的4天里，合成的5S RNA量急剧下降，并且在花粉成熟的最后48小时内，没有合成5S rRNA。