5S rRNA和18S（16S）rRNA 3'端附近存在的互补序列之间的分子间碱基配对相互作用可能参与核糖体亚基的可逆结合。
Intermolecular base-paired interaction between complementary sequences present near the 3' ends of 5S rRNA and 18S (16S) rRNA might be involved in the reversible association of ribosomal subunits.
作者信息
Azad A A
出版信息
Nucleic Acids Res. 1979 Dec 11;7(7):1913-29. doi: 10.1093/nar/7.7.1913.
Abstract
Highly conserved sequences present at an identical position near the 3' ends of eukaryotic and prokaryotic 5S rRNAs are complementary to the 5' strand of the m2(6)A hairpin structure near the 3' ends of 18S rRNA and 16S rRNA, respectively. The extent of base-pairing and the calculated stabilities of the hybrids that can be constructed between 5S rRNAs and the small ribosomal subunit RNAs are greater than most, if not all, RNA-RNA interactions that have been implicated in protein synthesis. The existence of complementary sequences in 5S rRNA and small ribosomal subunit RNA, along with the previous observation that there is very efficient and selective hybridization in vitro between 5S and 18S rRNA, suggests that base-pairing between 5S rRNA in the large ribosomal subunit and 18S (16S) rRNA in the small ribosomal subunit might be involved in the reversible association of ribosomal subunits. Structural and functional evidence supporting this hypothesis is discussed.
摘要
真核生物和原核生物5S rRNA 3'端附近相同位置存在的高度保守序列，分别与18S rRNA和16S rRNA 3'端附近m2(6)A发夹结构的5'链互补。5S rRNA与小核糖体亚基RNA之间可构建的杂交体的碱基配对程度和计算出的稳定性，大于（如果不是全部）大多数与蛋白质合成有关的RNA-RNA相互作用。5S rRNA和小核糖体亚基RNA中互补序列的存在，以及之前观察到的5S和18S rRNA在体外有非常高效和选择性的杂交现象，表明大核糖体亚基中的5S rRNA与小核糖体亚基中的18S（16S）rRNA之间的碱基配对可能参与核糖体亚基的可逆结合。本文讨论了支持这一假设的结构和功能证据。
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