Nakamura Harukazu, Nakano Kensuke E, Yasuda Mineo
Department of Anatomy, Hiroshima University School of Medicine, Kasumi 1-2-3, Minami-ku, Hiroshima 734, Japan.
Dev Growth Differ. 1986 Apr;28(2):185-190. doi: 10.1111/j.1440-169X.1986.00185.x.
The ontogeny of thymic myoid cells in the chick was studied electron microscopically and immunohistochemically. An anticreatine kinase antibody which reacts specifically to skeletal muscle cells was used. This antibody reacts only to myoid cells in the thymus. Myoid cells were found in the medulla or in the interlobular region, though the number of the myoid cells was small. Immunohistochemically, myoid cells were detected on the 18th day of incubation. Mature myoid cells showed clear cross striations after immunohistochemical staining around the time of hatching. Electron microscopically, myoid cells were detectable on the 19th day of incubation. The discrepancy between immunohistochemical and electron microscopical detection may be due to the low number of myoid cells.
利用电子显微镜和免疫组织化学方法对鸡胸腺肌样细胞的个体发生进行了研究。使用了一种对骨骼肌细胞具有特异性反应的抗肌酸激酶抗体。该抗体仅与胸腺中的肌样细胞发生反应。肌样细胞见于髓质或小叶间区域，不过其数量较少。免疫组织化学检测发现，孵育第18天时可检测到肌样细胞。在孵化前后进行免疫组织化学染色后，成熟肌样细胞显示出清晰的横纹。电子显微镜观察发现，孵育第19天时可检测到肌样细胞。免疫组织化学检测与电子显微镜检测结果存在差异可能是由于肌样细胞数量较少所致。
