Noguchi Motoko, Watanabe Chiaki, Kobayashi Takashi, Kuwashima Miho, Sakurai Takayuki, Katoh Hideki, Moriwaki Kazuo
Department of Biology, Faculty of Science, Shizuoka University, Ohya 836, Shizuoka 422, Japan.
Department of Genetics, Central Institute for Experimental Animals, Kawasaki 216, Japan.
Dev Growth Differ. 1996 Feb;38(1):59-69. doi: 10.1046/j.1440-169X.1996.00008.x.
The ter (teratoma) gene causes germ cell deficiency and a high incidence of congenital testicular teratomas derived from primordial germ cells in 129/Sv-ter strain mice. Ovarian teratomas in LTXBJ mice originate from ovarian parthenotes. In order to study the function of the ter gene in germ cell development and teratocarcinogenesis, we examined the influence of a foreign genetic background on the ter action by introducing the ter gene of 129/Sv-ter strain mice into C57BL/6J, LTXBJ and C3H/HeJ genetic backgrounds by the backcross method and by thus establishing B6-ter, LTXBJ-ter and C3H-ter ter congenic strains, respectively. Histological analysis showed that germ cell deficiency occurred in both sexes of the ter mutants, through the fetal stages to adulthood, but that congenital testicular teratocarcinogenesis did not occur after the fifth backcross generation. The ter/ter gonads were smaller than normal (+/+ or +/ter). Experimental testicular teratomas never developed from intratesticular grafts of B6-ter genital ridges. LTXBJ-ter/ter females had no ovarian teratomas. It is concluded that the ter gene is solely responsible for germ cell deficiency, but not testicular teratocarcinogenesis, in ter congenic strains having background genes other than 129/Sv-ter and that the ter gene is not involved in ovarian teratocarcinogenesis.
ter（畸胎瘤）基因会导致生殖细胞缺陷，并使129/Sv-ter品系小鼠中源自原始生殖细胞的先天性睾丸畸胎瘤发病率很高。LTXBJ小鼠的卵巢畸胎瘤起源于卵巢孤雌生殖细胞。为了研究ter基因在生殖细胞发育和畸胎癌发生中的作用，我们通过回交法将129/Sv-ter品系小鼠的ter基因导入C57BL/6J、LTXBJ和C3H/HeJ遗传背景中，从而分别建立了B6-ter、LTXBJ-ter和C3H-ter ter同源近交系，以此来检测外来遗传背景对ter基因作用的影响。组织学分析表明，ter突变体的两性从胎儿期到成年期均出现生殖细胞缺陷，但在第五代回交后未发生先天性睾丸畸胎癌。ter/ter性腺比正常的（+/+或+/ter）小。B6-ter生殖嵴的睾丸内移植从未形成实验性睾丸畸胎瘤。LTXBJ-ter/ter雌性小鼠没有卵巢畸胎瘤。得出的结论是，在具有129/Sv-ter以外背景基因的ter同源近交系中，ter基因是生殖细胞缺陷的唯一原因，但不是睾丸畸胎癌发生的原因，并且ter基因不参与卵巢畸胎癌的发生。
