Sakurai T, Katoh H, Moriwaki K, Noguchi T, Noguchi M
Department of Biology, Faculty of Science, Shizuoka University, Japan.
Mamm Genome. 1994 Jun;5(6):333-6. doi: 10.1007/BF00356550.
A single recessive gene, ter (teratoma), causes germ cell deficiency and a high incidence of congenital testicular teratomas in the 129/Sv-ter strain of the mouse. Linkage analyses between the ter gene and 36 marker genes of 19 chromosomes were performed with matings between the C57BL/6J-ter congenic strain and four inbred strains. Results showed that the ter gene was linked to D18Mit9, D18Mit14, and D18Mit17 on Chromosome (Chr) 18. Gene order estimated on the basis of recombination distance (in centimorgans) was [centromere-D18Mit14-5.1 (cM)-ter-0 (cM)-D18Mit17-23.8 (cM)-D18Mit9]. D18Mit17 is the microsatellite DNA of the Grl-1 (glucocorticoid receptor-1) locus. We conclude that the ter gene is closely linked to Grl-1 on Chr 18 and is a new mutation involving the developmental modification of primordial germ cells in mice.
一个隐性基因ter（畸胎瘤）会导致小鼠129/Sv-ter品系出现生殖细胞缺陷以及先天性睾丸畸胎瘤的高发病率。利用C57BL/6J-ter同基因品系与四个近交系进行交配，对ter基因与19条染色体上的36个标记基因进行了连锁分析。结果表明，ter基因与18号染色体上的D18Mit9、D18Mit14和D18Mit17连锁。根据重组距离（以厘摩为单位）估算的基因顺序为[着丝粒-D18Mit14-5.1（cM）-ter-0（cM）-D18Mit17-23.8（cM）-D18Mit9]。D18Mit17是Grl-1（糖皮质激素受体-1）基因座的微卫星DNA。我们得出结论，ter基因与18号染色体上的Grl-1紧密连锁，是一个涉及小鼠原始生殖细胞发育修饰的新突变。
