Yang Xiao-Yun, Gao Pei-Yun, Chen Xiao-Xu, Wang Li-Xia, Jiang Tong, Wu Tong, Chen Ying-Ying, Yue Chun-Yu, Wu Hong-Wei, Tang Li-Ying, Wang Zhu-Ju
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China School of Pharmacy, Henan University of Chinese Medicine Zhengzhou 450046, China.
Zhongguo Zhong Yao Za Zhi. 2023 May;48(9):2471-2479. doi: 10.19540/j.cnki.cjcmm.20230104.201.
In order to comprehensively evaluate the quality of Viticis Fructus, this study established HPLC fingerprints and evaluated the quality of 24 batches of Viticis Fructus samples from different species by similarity evaluation and multivariate statistical analysis(PCA, HCA, PLS-DA). On this basis, an HPLC method was established to compare the content differences of the main components, including casticin, agnuside, homoorientin, and p-hydroxybenzoic acid. The analysis was performed on the chromatographic column(Waters Symmetry C_(18)) with a gradient mobile phase of acetonitrile(A)-0.05% phosphoric acid solution(B) at the flow rate of 1 mL·min~(-1) and detection wavelength of 258 nm. The column temperature was 30 ℃ and the injection volume was 10 μL. The HPLC fingerprint of 24 batches of Viticis Fructus samples was established with 21 common peaks, and nine peaks were identified. Similarity analysis was carried out based on chromatographic data of 24 batches of chromatographic data of Viticis Fructus, and the results showed that except for DYMJ-16, the similarity of Vitex trifolia var. simplicifolia was ≥0.900, while that of V. trifolia was ≤0.864. In addition, the similarity analysis of two different species showed that the similarity of 16 batches of V. trifolia var. simplicifolia was 0.894-0.997 and that of the eight batches of V. trifolia was between 0.990 and 0.997. The results showed that the similarity of fingerprints of these two species was different, but the similarity between the same species was good. The results of the three multivariate statistical analyses were consistent, which could distinguish the two different species. The VIP analysis results of PLS-DA showed that casticin and agnuside contributed the most to the distinction. The content determination results showed that there was no significant difference in the content of homoorientin and p-hydroxybenzoic acid in Viticis Fructus from different species, but the content of casticin and agnuside was significantly different in different species(P<0.01). The content of casticin was higher in V. trifolia var. simplicifolia, while agnuside was higher in V. trifolia. The findings of this study show that there are differences in fingerprint similarity and component content of Viticis Fructus from different species, which can provide references for the in-depth study of the quality and clinical application of Viticis Fructus.
为全面评价蔓荆子的质量，本研究建立了高效液相色谱指纹图谱，并通过相似度评价和多元统计分析（主成分分析、聚类分析、偏最小二乘判别分析）对24批不同品种蔓荆子样品的质量进行评价。在此基础上，建立了高效液相色谱法比较主要成分包括紫花牡荆素、蔓荆子苷、异荭草素和对羟基苯甲酸的含量差异。分析采用Waters Symmetry C₁₈色谱柱，以乙腈（A）-0.05%磷酸溶液（B）为梯度流动相，流速为1 mL·min⁻¹，检测波长为258 nm。柱温为30℃，进样量为10 μL。建立了24批蔓荆子样品的高效液相色谱指纹图谱，共有21个共有峰，鉴定出9个峰。基于24批蔓荆子的色谱数据进行相似度分析，结果表明，除DYMJ-16外，单叶蔓荆的相似度≥0.900，而三叶蔓荆的相似度≤0.864。此外，两种不同品种的相似度分析表明，16批单叶蔓荆的相似度为0.894 - 0.997，8批三叶蔓荆的相似度在0.990至0.997之间。结果表明，这两个品种的指纹图谱相似度不同，但同一品种间的相似度良好。三种多元统计分析结果一致，能够区分这两个不同品种。偏最小二乘判别分析的VIP分析结果表明，紫花牡荆素和蔓荆子苷对区分贡献最大。含量测定结果表明，不同品种蔓荆子中异荭草素和对羟基苯甲酸的含量无显著差异，但不同品种间紫花牡荆素和蔓荆子苷的含量差异显著（P<0.01）。单叶蔓荆中紫花牡荆素含量较高，而三叶蔓荆中蔓荆子苷含量较高。本研究结果表明，不同品种蔓荆子的指纹图谱相似度和成分含量存在差异，可为蔓荆子质量的深入研究和临床应用提供参考。
