Morishima Ken, Inoue Rintaro, Sugiyama Masaaki
Institute for Integrated Radiation and Nuclear Science, Kyoto University, 2-1010 Asashironishi, Kumatori, Sennan-gun, Osaka 590-0494, Japan.
J Appl Crystallogr. 2023 Apr 25;56(Pt 3):624-632. doi: 10.1107/S1600576723002406. eCollection 2023 Jun 1.
Aggregates cause a fatal problem in the structural analysis of a biomacro-mol-ecule in solution using small-angle X-ray or neutron scattering (SAS): they deteriorate the scattering profile of the target molecule and lead to an incorrect structure. Recently, an integrated method of analytical ultracentrifugation (AUC) and SAS, abbreviated AUC-SAS, was developed as a new approach to overcome this problem. However, the original version of AUC-SAS does not offer a correct scattering profile of the target molecule when the weight fraction of aggregates is higher than 10%. In this study, the obstacle point in the original AUC-SAS approach is identified. The improved AUC-SAS method is then applicable to a solution with a relatively larger weight fraction of aggregates (≤20%).
聚集体在使用小角X射线或中子散射（SAS）对溶液中的生物大分子进行结构分析时会引发一个致命问题：它们会破坏目标分子的散射图谱并导致结构错误。最近，一种将分析超速离心法（AUC）和SAS相结合的方法（简称为AUC-SAS）被开发出来，作为克服这一问题的新途径。然而，当聚集体的重量分数高于10%时，原始版本的AUC-SAS无法提供目标分子的正确散射图谱。在本研究中，确定了原始AUC-SAS方法中的障碍点。改进后的AUC-SAS方法随后可应用于聚集体重量分数相对较大（≤20%）的溶液。
