LeBlanc Nicholas, Gebben Samantha
United States Department of Agriculture, Agricultural Research Service, Crop Improvement and Protection Research Unit Salinas California USA.
Plant Environ Interact. 2023 Jan 11;4(1):11-22. doi: 10.1002/pei3.10099. eCollection 2023 Feb.
Bacterial communities play multiple functional roles in soil that have positive and negative feedbacks on plant health. However, relatively few studies have focused on the ecology of soil bacterial communities in commercial strawberry production systems. The objective of this study was to determine if ecological processes influencing soil bacterial communities are consistent among commercial strawberry production locations and plots within the same geographic region. Soil samples were collected using a spatially explicit design from three plots in two commercial strawberry production locations in the Salinas Valley region of California. Soil carbon, nitrogen, and pH were measured for each of the 72 soil samples and bacterial communities were characterized using 16 S rRNA sequencing. Multivariate analyses showed bacterial community composition was differentiated between the two strawberry production locations. Analyses of communities within plots demonstrated soil pH and nitrogen were significant predictors of bacterial community composition in one of the three sampled plots. Bacterial communities displayed spatial structure in two plots at one location based on a significant increase in community dissimilarity with increasing spatial distance. Null model analyses identified a lack of phylogenetic turnover among bacterial communities in all plots, but a greater frequency of dispersal limitation in the two plots where spatial structure was also observed. Overall, this work suggests that ecological factors influencing soil bacterial communities are not consistent among different strawberry production locations or plots which may impact the ability to predict or manage the effect of soil microbiomes on strawberry health.
细菌群落对土壤起着多种功能作用，这些作用对植物健康有着正负反馈。然而，相对较少的研究关注商业草莓生产系统中土壤细菌群落的生态学。本研究的目的是确定影响土壤细菌群落的生态过程在商业草莓生产地点以及同一地理区域内的地块之间是否一致。在加利福尼亚州萨利纳斯谷地区的两个商业草莓生产地点，从三个地块采用空间明确的设计采集土壤样本。对72个土壤样本中的每一个都测量了土壤碳、氮和pH值，并使用16S rRNA测序对细菌群落进行了表征。多变量分析表明，两个草莓生产地点之间细菌群落组成存在差异。对地块内群落的分析表明，土壤pH值和氮是三个采样地块之一中细菌群落组成的重要预测因子。基于群落差异随空间距离增加而显著增加，一个地点的两个地块中的细菌群落呈现出空间结构。空模型分析表明，所有地块中的细菌群落之间缺乏系统发育周转，但在也观察到空间结构的两个地块中，扩散限制的频率更高。总体而言，这项工作表明，影响土壤细菌群落的生态因素在不同的草莓生产地点或地块之间并不一致，这可能会影响预测或管理土壤微生物群对草莓健康影响的能力。
