Energy-rich Compounds Production by Photosynthetic Carbon Fixation Research Center, Shandong Key Lab of Applied Mycology, College of Life Sciences, Qingdao Agricultural University, Qingdao, 266109, Shandong Province, People's Republic of China; Shandong Key Laboratory of Environmental Processes and Health, School of Environmental Science and Engineering, Shandong University, Qingdao, 266237, Shandong Province, People's Republic of China.
Shandong Key Laboratory of Environmental Processes and Health, School of Environmental Science and Engineering, Shandong University, Qingdao, 266237, Shandong Province, People's Republic of China.
Biotechnol Adv. 2023 Oct;67:108183. doi: 10.1016/j.biotechadv.2023.108183. Epub 2023 Jun 5.
Elevated CO emissions have substantially altered the worldwide climate, while the excessive reliance on fossil fuels has exacerbated the energy crisis. Therefore, the conversion of CO into fuel, petroleum-based derivatives, drug precursors, and other value-added products is expected. Cupriavidus necator H16 is the model organism of the "Knallgas" bacterium and is considered to be a microbial cell factory as it can convert CO into various value-added products. However, the development and application of C. necator H16 cell factories has several limitations, including low efficiency, high cost, and safety concerns arising from the autotrophic metabolic characteristics of the strains. In this review, we first considered the autotrophic metabolic characteristics of C. necator H16, and then categorized and summarized the resulting problems. We also provided a detailed discussion of some corresponding strategies concerning metabolic engineering, trophic models, and cultivation mode. Finally, we provided several suggestions for improving and combining them. This review might help in the research and application of the conversion of CO into value-added products in C. necator H16 cell factories.
CO 排放的增加已极大地改变了全球气候，而对化石燃料的过度依赖又加剧了能源危机。因此，预计将 CO 转化为燃料、石油衍生产品、药物前体和其他附加值产品。恶臭假单胞菌 H16 是“Knallgas”细菌的模式生物，被认为是微生物细胞工厂，因为它可以将 CO 转化为各种附加值产品。然而，恶臭假单胞菌 H16 细胞工厂的开发和应用存在一些局限性，包括效率低、成本高以及菌株自养代谢特性所带来的安全问题。在这篇综述中，我们首先考虑了恶臭假单胞菌 H16 的自养代谢特性，然后对由此产生的问题进行了分类和总结。我们还详细讨论了一些关于代谢工程、营养模型和培养模式的相应策略。最后，我们提出了一些改进和组合的建议。本文的综述可能有助于恶臭假单胞菌 H16 细胞工厂中 CO 转化为附加值产品的研究和应用。
